Abstract: The extraction process of flavonoid compounds originated from Valeriana ofecnilis( produced from Guizhou province) was investigated using ethanol as the extraction solvent. Method: Four factors,including concentration of Ethanol,ratio of raw material to solvent,extracting temperature and time,were optimized by response surface method.The optimum conditions were as follows: the concentration of ethanol was 53.0%; ratio of material to solvent was 1 ∶ 29 g / m L; extraction temperature was 73 ℃ and extraction time was 26 h. Under the optimum conditions,the difference of between real value of 2.133% and theoretic value in rate of extraction was about 3.5%. Conclusion: Response surface method was a reliable tool to optimize the extraction of flavonoid compounds from Valeriana ofecnilis.