• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
李静, 王瑶, 邓毛程. 泡菜中优良乳酸菌筛选及特性的研究[J]. 食品工业科技, 2016, (06): 229-232. DOI: 10.13386/j.issn1002-0306.2016.06.039
引用本文: 李静, 王瑶, 邓毛程. 泡菜中优良乳酸菌筛选及特性的研究[J]. 食品工业科技, 2016, (06): 229-232. DOI: 10.13386/j.issn1002-0306.2016.06.039
LI Jing, WANG Yao, DENG Mao-cheng. Screening and characterization of an excellent Lactobacillus isolated from pickle[J]. Science and Technology of Food Industry, 2016, (06): 229-232. DOI: 10.13386/j.issn1002-0306.2016.06.039
Citation: LI Jing, WANG Yao, DENG Mao-cheng. Screening and characterization of an excellent Lactobacillus isolated from pickle[J]. Science and Technology of Food Industry, 2016, (06): 229-232. DOI: 10.13386/j.issn1002-0306.2016.06.039

泡菜中优良乳酸菌筛选及特性的研究

Screening and characterization of an excellent Lactobacillus isolated from pickle

  • 摘要: 为了获得适用于纯种强化发酵泡菜生产的优良菌种,采用平板筛选法和发酵筛选法从泡菜中分离、筛选乳酸菌,采用形态、生理生化和16S rDNA等特性的分析技术对优选菌种进行鉴定,并通过发酵实验进一步研究优选菌种的发酵特性。经筛选,获得一株乳酸产量最高的菌种LP-09,该菌种被鉴定为植物乳杆菌(Lactobacillus plantarum)。发酵实验结果表明,菌种LP-09的最适温度和初始p H分别为37℃和6.0,具有较强的耐盐性,在含有40 g/L Na Cl的培养基中生长正常,在含有100 g/L Na Cl的培养基中可缓慢发酵产酸;同时,菌种LP-09具有较强的亚硝酸盐降解能力,对200 mg/L亚硝酸盐的降解率可达99.3%以上。 

     

    Abstract: To obtain excellent and pure breed strain suitable for producing fermented vegetable products,strains were isolated,screened from pickle using plate screening as well as fermentation screening method. And then,optimizing species were identified using morphological,physiological and biochemical properties and 16 S rDNA analysis technology. Finally,further study on fermentation characteristics of the target strain carried out a series of fermentation test. A strain named LP-09,with maximum lactic acid-producing,was isolated from pickle and identified as Lactobacillus plantarum. Results of fermentation tests showed that the optimal temperature and initial p H suitable for strain LP-09 were 37 ℃ and 6.0,respectively. Meanwhile,the strain owned better salt tolerance because it could grow normally in culture media with 40 g/L Na Cl,or could keep fermenting and producing lactic acid even at 100 g/L Na Cl concentration continuously and slowly. At the same time,strain LP-09 possessed quite strong ability to degrade nitrite and degradation rate of 200 mg/L nitrite could reach more than 99.3%.

     

/

返回文章
返回