Abstract: Solid lipid nanoparticles formulation was prepared by the combination of curcumin with tea polyphenols through film- ultrasonic wave dissolving techniques,a method for determining the five active content from curcumin and tea polyphenols in nanoparticles by HPLC- DAD was established,the stability of CUR- TP- SLNs under different storage conditions and evaluate the anti- tumor activity were studied.The results showed that the optimum formula was selected as curcumin and tea polyphenols( 40 mg),stearic acid( 120 mg),lecithin( 60 mg) and tween-80( 1.0%,10 m L),the mean particle size diameter and polydispersity index( PDI) were 93.8 nm and 0.172,zeta potential was-44.5 m V,the average entrapment efficiency and the ratio of loading drag approached 93.08% and12.81%,respectively. The CUR- TP- SLNs formulation was stable under 4 ℃ storage.MTT assay showed that compared with curcumin and tea polyphenols,combined nanoparticles exhibited stronger inhibitory effect to Hep G2 and A549 cells( p < 0.05). To conclusion,the preparation technique of CUR- TP- SLNs was feasible and applicable with high entrapment efficiency,unique size and good stability,the curcumin and tea polyphenols clearly exhibit synergism effect against tumor cells activity. The combination of two phenolic components such as curcumin and tea polyphenols may have great potential in food industry.