Abstract: Antimicrobial peptide( AMP) is a kind of small peptides. AMP has the inhibition of various bacteria,fungi and viruses. However,the low quantity of peptides and complex purification process are still a remarkable bottleneck for scientific and industrial research development. Heterologous expression technology provides a effective way to improve the production of AMP. The main heterologous systems currently used for AMP production,including bacteria,yeast. This review described the heterologous expression system of antimicrobial peptide,compared the advantages and disadvantages in each expression system.It was designed to provide the basis of theoretical and technical support for the mass production of antimicrobial peptides by biotechnological tools.