Abstract: Oxford cup and double dilution methods were used to study the anti- bacterial activities of old white tea on Staphylococcus aureus and Shigella. To discuss the correlation between biochemical components and antibacterial circle diameter.Results showed that the correlation between year and anti- bacterial activities reached significant level,the anti- bacterial effect of white tea produced lately was the best.The antibacterial ability showed a trend of decrease with the extension of storage time,through path analysis,the linear regression equation of main biochemical components and Staphylococcus aureus antibacterial circle the diameter was got,Y₁= 30.121 + 0.818X₃( tea polyphenols)- 6.798 X₄( caffeine) + 0.753 X₅( flavones) + 0.296 X₆( organic acids). The linear regression equation of main biochemical components and Shigella antibacterial circle the diameter was got,Y₂= 1.996-0.173X₁( water extract) + 0.384 X₅( flavones) + 0.831 X₇( color L*). The equations show that main biochemical components play important roles in anti- bacterial activities of white tea.