钙离子通道抑制剂处理下发芽大豆主要生理生化和γ-氨基丁酸的代谢变化
Physiological change and the regulation of γ-aminobutyric acid accumulation in germinated soybean under inhibitors of calcium channel treatment
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摘要: 为研究钙离子通道抑制剂对Na Cl胁迫下发芽大豆生理代谢和γ-氨基丁酸（GABA）富集的影响,通过Na Cl联合质膜钙离子通道抑制剂（La Cl3）和内膜钙离子通道抑制剂（Heparin）处理大豆籽粒,分析发芽期间主要生理生化和GABA代谢酶活力的变化。结果显示,发芽大豆经Na Cl联合La Cl3处理后,其生长发育较单独Na Cl处理进一步受到抑制,芽长显著降低,经Heparin处理芽长无显著性变化,而二者呼吸速率均显著增加。相较单独Na Cl处理,Na Cl联合La Cl3或Heparin处理后其子叶中GABA代谢酶活力均显著下降,La Cl3处理后子叶和胚中GABA含量分别为Na Cl处理的50%和79%,而Heparin处理则分别为70%和66%,表明抑制内源钙库的释放对Na Cl胁迫下GABA富集的影响小于抑制质膜钙离子通道。Abstract: The effect of membrane calcium channel inhibitor( La Cl3) and endometrium calcium channel inhibitor( Heparin) on the main physiological metabolism and γ- aminobutyric acid( GABA) accumulation in soybeans during germination under Na Cl stress were investigated. Under Na Cl combining La Cl3 treatment,the growth of germinating soybeans was further suppressed,the sprout length decreased significantly,while no significant change was observed under heparin treatment. However,the respiratory rate both increased significantly under La Cl3 and heparin treatment.Compared with Na Cl treatment,rate- limiting enzyme activities of GABA metabolism in cotyledon decreased significantly under Na Cl plus La Cl3 or heparin treatment.Under La Cl3 treatment,GABA content was 50% and 79% of the Na Cl treatment in cotyledon and embryo,respectively.However,under heparin treatment,GABA content in cotyledon and embryo was 70% and 66% of the Na Cl treatment,respectively.This result indicates that the inhibit effect of endogenous calcium stores on GABA content was smaller than membrane calcium channel.
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