Abstract: Twelve strains were isolated from 5 samples of traditional fermented dairy tofu in Horqin Region.Sequence analysis of 16 S rDNA after PCR amplification and construct a phylogeny tree was used for the identification of 12 strains. The results demonstrated that the 12 strains were all the Lactic acid bacteria strains,including 6Lactobacillus strains and 6 Lactococcus strains were respectively: Lactobacillus plantarum( 4 strains),Lactobacillus casei( 1strain),Lactobacillus pentosus( 1 strain),Pediococcus acidilactici( 5 strains),Pediococcus pentosaceus( 1strain).The results of sequence analysis of 16 S r DNA were in accordance with that of physiological- biochemical tests,Pediococcus acidilactici and Lactobacillus plantarum were dominant microfloras.