Abstract: Based on the actual situation in the transport process, the effects of mechanical vibration frequencies and acceleration on the storage quality of tomato fruit during the transportation process have been experimentally researched. The change processes of hardness, the content in internal soluble solids, titratable acid, VC, MDA under five gradation vibration frequencies of 10、20、30、40、50 Hz and three vibration accelerations of 2.5, 5, 7.5 m/s2 have been researched.The results indicated that the hardness, titratable acid, VCwere decreased whereas the content of MDA were increased during the time of experiment, and the content of internal soluble solids decreased after a few days increased.It also can be found that 20 Hz vibration frequencies and2.5 m/s2 vibration accelerations is the least loss damage condition for tomato fruit storage quality during the transportation process.