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中国精品科技期刊2020
周志阳, 曹有龙, 王艺涵, 陈帝君, 米佳, 李晓莺, 闫亚美, 曾晓雄. 枸杞芽茶与叶茶的化学成分和抗氧化活性分析[J]. 食品工业科技, 2017, (10): 129-134. DOI: 10.13386/j.issn1002-0306.2017.10.017
引用本文: 周志阳, 曹有龙, 王艺涵, 陈帝君, 米佳, 李晓莺, 闫亚美, 曾晓雄. 枸杞芽茶与叶茶的化学成分和抗氧化活性分析[J]. 食品工业科技, 2017, (10): 129-134. DOI: 10.13386/j.issn1002-0306.2017.10.017
ZHOU Zhi-yang, CAO You-long, WANG Yi-han, CHENG Di-jun, MI Jia, LI Xiao-ying, YAN Ya-mei, ZENG Xiao-xiong. Analysis of chemical compositions and antioxidant activities of Lycium barbarum bud and leaf teas[J]. Science and Technology of Food Industry, 2017, (10): 129-134. DOI: 10.13386/j.issn1002-0306.2017.10.017
Citation: ZHOU Zhi-yang, CAO You-long, WANG Yi-han, CHENG Di-jun, MI Jia, LI Xiao-ying, YAN Ya-mei, ZENG Xiao-xiong. Analysis of chemical compositions and antioxidant activities of Lycium barbarum bud and leaf teas[J]. Science and Technology of Food Industry, 2017, (10): 129-134. DOI: 10.13386/j.issn1002-0306.2017.10.017

枸杞芽茶与叶茶的化学成分和抗氧化活性分析

Analysis of chemical compositions and antioxidant activities of Lycium barbarum bud and leaf teas

  • 摘要: 以宁夏枸杞芽茶、枸杞叶茶为原料,利用热水浸提(料液比1∶40、温度96℃、时间30 min)制备枸杞芽茶与叶茶提取物并对其所含的化学成分含量进行测定,采用HPLC对枸杞芽茶与叶茶提取物中游离氨基酸的组成进行了分析,利用DPPH自由基、ABTS自由基、超氧阴离子自由基清除率、总还原力为指标对枸杞芽茶、叶茶茶汤提取物的抗氧化活性进行分析评价。结果表明,枸杞芽茶、枸杞叶茶提取物中多酚含量分别为6.32%、2.96%。枸杞芽茶与叶茶提取物中天冬氨酸(3230.41、2440.96μg/g)、天冬酰胺(3754.77、3495.57μg/g)、组氨酸(5585.26、2825.17μg/g)以及亮氨酸(4376.09、1051.00μg/g)含量较为丰富。枸杞芽茶与叶茶提取物相比抗氧化活性较强,二者对DPPH自由基(IC50:147.63、241.23μg/m L)、ABTS自由基(IC50:109.87、145.32μg/m L)以及超氧阴离子自由基(IC50:46.58、54.32μg/m L)均具有良好的清除能力,与叶茶相比,枸杞芽茶具有更好的抗氧化活性。 

     

    Abstract: The extracts of Lycium barbarum bud and leaf teas were prepared by hot water extraction ( ratio of solid to liquid1∶ 40, temperature 96 ℃, extraction time 30 min) and their chemical contents were determined, and compositions of free amino acids in Lycium barbarum bud and leaf teas were analyzed by HPLC.Furthermore, the antioxidant activities of Lycium barbarum bud and leaf tea were evaluated by determining of scavenging activities on DPPH radical, ABTS radical and superoxide radical and total reducing power.The results showed that the total polyphenols contents for Lycium barbarum bud and leaf teas were6.32% and 2.96%, respectively. Asp ( 3230.41 and 2440.96 μg/g) , Asn ( 3754.77 and 3495.57 μg/g) , His ( 5585.26 and2825.17 μg/g) and Leu ( 4376.09 and 1051.00 μg/g) were relatively abundant in Lycium barbarum bud and leaf teas.Moreover, both Lycium barbarum bud tea and leaf tea exhibited favorable scavenging activities on DPPH ( IC50: 147.63 and 241.23 μg/m L) , ABTS ( IC50: 109.87 and 145.32 μg/m L) and superoxide radicals ( IC50: 46.58 and 54.32 μg/m L) . Compared with Lycium barbarum leaf tea, Lycium barbarum bud tea showed relatively higher antioxidant activity.

     

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