Abstract: To isolate and purify β-1, 3-glucosidase from Perinereis aibuhitensis and study its properties, the crude β-1, 3-glucosidase was obtained from the P.aibuhitensis by ammonium sulfate precipitation, which was purified using ion-exchange chromatography on DEAE-52 and gel filtration chromatography on Sephadex G-100. Finally the β-1, 3-glucosidase was purified 35.74-fold and 39.49% recovery yield. The β-1, 3-glucosidase was estimated to be 28.7 k Da with SDS-PAGE. The optimal temperature for the β-1, 3-glucosidase was 50 ℃.The optimum p H was 7.The Kmand Vmaxof β-1, 3-glucosidase were8.2 × 10-4mol/L and 3.2 × 10-4μmol/h, respectively.The enzyme activity was not affected by K+, Mg²⁺, Fe²⁺, Ba²⁺and Ca²⁺, while inactivated by Al³⁺, Cu²⁺, Zn²⁺and Ag⁺, and especially strongly inactivated by Zn²⁺. P. aibuhitensis might be a candidate resource in producing β-1, 3-glucosidase.