Abstract: The extraction of total alkaloids from Holarrhena antidysenteriaca was optimized.Single factor experiment was employed to identify four main process parameters.The selected variables were optimized by response surface analysis based on the extraction yield of total alkaloids.The results showed that the effect of the investigated factors on the total alkaloids extraction yield was in the order as: extraction temperature > ethanol concentration > extraction time > matedal-to-solven ratio.The optimal extraction parameters were 85% ethanol and subsequent extraction at 80 ℃ for 3 h with a matedal-to-solven ratio1∶ 10 g/m L. The extraction yield of total alkaloids obtained was ( 23.8 ± 0.12) mg/g under these conditions.This described method was reliable.Highly extraction and suitable for the extraction of total alkaloids from Holarrhena antidysenteriaca.