Abstract: The model of tertiary of β-conglycinin beta-subunit was constructed by the bioinformation tool SWISS-MODEL, and the conformational epitopes were predicted by Disco Tope 2.0 server.The overlapping fragments of β-conglycinin beta-subunit genes were amplified by PCR and inserted into p MD-18 T vector, then preserved into competent cell of Escherichia coli JM109.The recombinant plasmids were evaluated by blue-white selection, PCR and double enzyme digestion, and DNA sequence analysis showed that the cloned sequence was the very fragment designed. The successful cloning of the genes can provide a reference for the study of the expresstion of β-conglycinin beta-subunit and screening of antigenic epitopes.