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中国精品科技期刊2020
吴红, 周佺, 郭善广, 万俊, 吴炜亮, 蒋爱民, 罗碧琳, 祁焰晶. 不同杀菌方式对软质胶原蛋白冻品质及抗氧化活性的影响[J]. 食品工业科技, 2017, (19): 52-56. DOI: 10.13386/j.issn1002-0306.2017.19.010
引用本文: 吴红, 周佺, 郭善广, 万俊, 吴炜亮, 蒋爱民, 罗碧琳, 祁焰晶. 不同杀菌方式对软质胶原蛋白冻品质及抗氧化活性的影响[J]. 食品工业科技, 2017, (19): 52-56. DOI: 10.13386/j.issn1002-0306.2017.19.010
WU Hong, ZHOU Quan, GUO Shan-guang, WAN Jun, WU Wei-liang, JIANG Ai-min, LUO Bi-lin, QI Yan-jing. Effects of different sterilization methods on the quality and antioxidant activity of soft collagen jelly[J]. Science and Technology of Food Industry, 2017, (19): 52-56. DOI: 10.13386/j.issn1002-0306.2017.19.010
Citation: WU Hong, ZHOU Quan, GUO Shan-guang, WAN Jun, WU Wei-liang, JIANG Ai-min, LUO Bi-lin, QI Yan-jing. Effects of different sterilization methods on the quality and antioxidant activity of soft collagen jelly[J]. Science and Technology of Food Industry, 2017, (19): 52-56. DOI: 10.13386/j.issn1002-0306.2017.19.010

不同杀菌方式对软质胶原蛋白冻品质及抗氧化活性的影响

Effects of different sterilization methods on the quality and antioxidant activity of soft collagen jelly

  • 摘要: 以猪皮胶原蛋白酶解液为主要原料,经热诱导凝胶制成软质胶原蛋白冻,系统研究不同的杀菌方式(巴氏杀菌、常压沸水杀菌、高压蒸汽杀菌)对其品质及抗氧化特性的影响。研究结果表明,高压蒸汽杀菌后软质胶原蛋白冻的色泽变黄,硬度、弹性和咀嚼性都明显下降(p<0.05)。不同杀菌方式处理后,经超滤分离的四个组分(>50、10~50、5~10、<5 k Da)中,分子量>50 k Da的蛋白质组分所占比例最大,常压沸水杀菌组中分子量为5~10 k Da和<5 k Da的组分比例最大,分别为25.71%和9.48%。常压沸水杀菌后软质胶原蛋白冻的DPPH自由基和羟基自由基的清除能力最强,其IC50分别为0.042 g/m L和0.093 g/m L,且色泽明亮且弹性较好。常压沸水杀菌不仅有良好的杀菌效果,还能较好的保持软质胶原蛋白冻的质构特性和抗氧化活性,这为新型软质胶原蛋白的冻加工技术奠定基础。 

     

    Abstract: In this research, pigskin collagen enzyme solution was used as main raw material to make a soft collagen jelly, on the basis of this, the effects of different sterilization methods ( pasteurization, atmospheric pressure boiling water sterilization, high pressure steam sterilization) on the quality and antioxidant activity of soft collagen gel were investigated.Results showed that the color of soft collagen jelly with high pressure steam sterilization was yellow, the hardness, flexibility and chewiness were decreased significantly ( p < 0.05) .After different sterilization process, there were four protein components separated by ultrafiltration ( > 50, 1050, 510, < 5 k Da) , and macromolecular protein ( > 50 k Da) accounted for the biggest percentage, the content of protein which were 510 k Da and < 5 k Da of atmospheric pressure boiling water sterilization were highest, accounted for 25.71% and9.48%, the scavenging activity of DPPH· and ·OH was strongest in atmospheric pressure boiling water sterilization group, IC50 were 0.042 g/m L and 0.093 g/m L, and the soft collagen jelly showed a bright colour and good elasticity.In conclusion, atmospheric pressure boiling water sterilization not only has good sterilization effect, also can keep soft collagen jelly's texture and antioxidant activity better, which lay the foundation for the new soft collagen jelly's processing technology.

     

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