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中国精品科技期刊2020
李远宏, 姜华, 焦阳, 徐湾, 邹小倩, 裴尚飞. 食品香辛料和调味品中克罗诺杆菌的分离与鉴定[J]. 食品工业科技, 2017, (19): 125-130. DOI: 10.13386/j.issn1002-0306.2017.19.024
引用本文: 李远宏, 姜华, 焦阳, 徐湾, 邹小倩, 裴尚飞. 食品香辛料和调味品中克罗诺杆菌的分离与鉴定[J]. 食品工业科技, 2017, (19): 125-130. DOI: 10.13386/j.issn1002-0306.2017.19.024
LI Yuan-hong, JIANG Hua, JIAO Yang, XU Wan, ZOU Xiao-qian, PEI Shang-fei. Isolation and identification of Cronobacter spp.from dried spices and condiments[J]. Science and Technology of Food Industry, 2017, (19): 125-130. DOI: 10.13386/j.issn1002-0306.2017.19.024
Citation: LI Yuan-hong, JIANG Hua, JIAO Yang, XU Wan, ZOU Xiao-qian, PEI Shang-fei. Isolation and identification of Cronobacter spp.from dried spices and condiments[J]. Science and Technology of Food Industry, 2017, (19): 125-130. DOI: 10.13386/j.issn1002-0306.2017.19.024

食品香辛料和调味品中克罗诺杆菌的分离与鉴定

Isolation and identification of Cronobacter spp.from dried spices and condiments

  • 摘要: 利用生理生化鉴定方法和分子生物学方法对从湖南、湖北、江苏和上海等地的8个地区采集的64份干制食品香辛料和调味品样品进行了克罗诺杆菌分离与鉴定,结果从19份样品中检出了克罗诺杆菌,污染率为29.7%,其中检出率较高的样品为白胡椒粉、花椒粉和红辣椒粉,污染率分别为62.5%、50.0%和33.3%。本研究从采样的7个地区的样品中检出了克罗诺杆菌,其中检出率最高的地区为湖南永州,其次为上海市和湖北赤壁,检出率分别为50.0%、37.5%和33.3%。利用基于fus A基因序列分析的方法对克罗诺杆菌分离株进行了种的水平的鉴定,结果将本研究分离得到的克罗诺杆菌鉴定为5个种,其中9株(47.4%)被鉴定为阪崎克罗诺杆菌(C.sakazakii),4株(21.0%)为苏黎世克罗诺杆菌(C.turicensis),3株(15.8%)为都柏林克罗诺杆菌(C.dublinensis),2株(10.5%)为丙二酸盐阳性克罗诺杆菌(C.malonaticus),1株(5.3%)为尤尼沃斯克罗诺杆菌(C.universalis)。研究结果表明市售干制食品香辛料和调味品中存在克罗诺杆菌的污染,应该加强对该类食品中克罗诺杆菌的流行病学监测。 

     

    Abstract: A total of sixty-four dried spices and condiments, collected from retail markets in 8 cities of Hunan, Hubei and Jiangsu province, as well as Shanghai, were analyzed for the presence of Cronobacter spp.In total, 19 ( 29.7%) of 64 dried spices and condiments were positive for Cronobacter spp., with the highest percentage ( 62.5%) coming from white pepper powder, followed by Chinese prickly ash powder ( 50.0%) and red chilli powder ( 33.3%) .Dried spices and condiments samples from 7cities were found to be contaminated with Cronobacter spp., with highest detection rate for Yongzhou, Hunan ( 50%) , followed by Shanghai ( 37.5%) and Chibi, Hubei ( 33.3%) .Based on the fus A gene sequence analysis, Cronobacter isolates were divided into 5 Cronobacter species. Most of the Cronobacter isolates were identified as C. sakazakii ( 9/19, 47.4%) , followed by C.turicensis ( 4/19, 21.0%) , C.dublinensis ( 3/19, 15.8%) and C. malonaticus ( 2/19, 10.5%) , while the remaining isolate ( 1/19, 5.3%) was identified as C. universalis. The results indicated that commercially available dried spices and condiments were possible reservoir of Cronobacter spp., and therefore the epidemiological surveillance of Cronobacter spp.should be strengthened in dried spices and condiments.

     

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