Abstract: Postharvest fruit & vegetable are perishable, therefore the preservation technology is indispensable to maintain quality and prolong shelf life.Chemical preservatives are effective to maintain the quality of postharvest fruit & vegetable, however there are health problem, environmental problem, drug resistance and so on.As a kind of safe, effective, eco-friendly fruit & vegetable preservation technology, bio-preservation technology based on microorganism is becoming research hotspot gradually. This technology is considered as an effective means to replace chemical preservatives and meet people's new requirement of natural and safe fruit & vegetable. This article summarizes the state of art in application and research of microorganism in fruit &vegetable storage, and discusses the problems and the future development.In order to provide references to extend application of microorganism in fruit & vegetable industry.