基因组改组快速提高日本小球藻脂肪产量

Increase the lipid production of Chlorella hirataii rapidly by genome shuffling

摘要: 以日本小球藻为出发藻株,经过紫外线和甲基磺酸乙酯分别诱变处理,获得4株总脂产量有所提高的突变株。以聚乙二醇作为融合剂,对获得的突变株进行两轮递归式原生质体融合,筛选到遗传稳定的改组藻株F2C2,其总脂含量为59.01%,较原始藻株提高了101.4%。对日本小球藻的原始藻株和改组藻株F2C2的油脂含量进行分析,结果表明改组前后日本小球藻的总脂组成成分没有变化,但各组分含量有较大差别。

Abstract: Strain Chlorella hirataii was used as the starting strains for genome shuffling. They were mutated by UV-light and ethylmesylate separately, and four mutant strains with increased lipids yield were selected.Two rounds of genome shuffling were carried out with the four mutant strains using PEG to mediate protoplasts fusion. Finally, the Chlorella hirataii F2 C2 was selected which produced lipids ( 59.01%) higher than the original strain by 101.4%. Compare with the original strain in the same batch, the total lipid composition of the Chlorella hirataii F2 C2 didn't change a lot, but there was a big gap between the content of each component.