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中国精品科技期刊2020
张杉杉, 涂传海, 肖愈, 周思多, 邢广良, 夏海燕, 张波, 董明盛. 蛹虫草固态发酵豆渣的功能性成分与抗氧化活性[J]. 食品工业科技, 2017, (24): 69-74. DOI: 10.13386/j.issn1002-0306.2017.24.014
引用本文: 张杉杉, 涂传海, 肖愈, 周思多, 邢广良, 夏海燕, 张波, 董明盛. 蛹虫草固态发酵豆渣的功能性成分与抗氧化活性[J]. 食品工业科技, 2017, (24): 69-74. DOI: 10.13386/j.issn1002-0306.2017.24.014
ZHANG Shan-shan, TU Chuan-hai, XIAO Yu, ZHOU Si-duo, XING Guang-liang, XIA Hai-yan, ZHANG Bo, DONG Ming-sheng. Functional components and antioxidant activity of okara fermented by Cordyceps militaris[J]. Science and Technology of Food Industry, 2017, (24): 69-74. DOI: 10.13386/j.issn1002-0306.2017.24.014
Citation: ZHANG Shan-shan, TU Chuan-hai, XIAO Yu, ZHOU Si-duo, XING Guang-liang, XIA Hai-yan, ZHANG Bo, DONG Ming-sheng. Functional components and antioxidant activity of okara fermented by Cordyceps militaris[J]. Science and Technology of Food Industry, 2017, (24): 69-74. DOI: 10.13386/j.issn1002-0306.2017.24.014

蛹虫草固态发酵豆渣的功能性成分与抗氧化活性

Functional components and antioxidant activity of okara fermented by Cordyceps militaris

  • 摘要: 目的:用蛹虫草固态发酵豆渣,测定豆渣发酵前后功能性成分含量及抗氧化能力的变化。方法:蛹虫草CM-1固态发酵豆渣7 d,测定发酵前后豆渣水提物中虫草素、总酚和多糖的含量,以及DPPH自由基清除力、还原力、ABTS自由基清除能力、铁离子还原力(FRAP)的变化,并用皮尔森相关性分析以及主成分分析研究了虫草素、总酚、多糖含量与抗氧化指标之间的关系。结果:豆渣经蛹虫草CM-1固态发酵,水提物中多糖、虫草素和总酚含量分别是(236.16±39.91)、(1.88±0.04)μg/mg和(11.94±0.33)μg GAE/mg,多糖、总酚含量分别是发酵前的1.42倍和2.15倍。豆渣经过发酵,在提取物浓度为6 mg/m L时,DPPH自由基清除力由24.63%提高到72.4%,还原力由0.11提高到0.38,ABTS自由基清除能力由76.76%提高至88.71%,FRAP由39.08μmol/L提高至269.55μmol/L。主成分分析和相关性分析表明,抗氧化指标与虫草素、总酚、多糖含量具有非常显著的相关性(p<0.01)。结论:用蛹虫草发酵豆渣可以提高豆渣的功能性成分和抗氧化能力,发酵豆渣有望开发成为功能性食品基料及抗氧化食品。 

     

    Abstract: Cordyceps militaris was used to fermented okara in solid state fermentation, and the change in functional ingredients and the antioxidant activity of fermented okara was tested.Okara was fermented by Cordyceps militaris for 7 days, the content of cordycepin, total phenlic and polysaccharide were tested. In addition, DPPH scavenging activity, reducing power, fluorescence recovery after photobleaching ( FRAP) and ABTS+·scavenging activity were tested to study the antioxidant activity of fermented okara.The relation between functional ingredients and antioxidant index were researched by the correlation analysis and principal component analysis.Results showed that the content of polysaccharide, cordycepin and total phenolic of water extracts were ( 236.16 ± 39.91) , ( 1.88 ± 0.04) μg/mg and ( 11.94 ± 0.33) μg GAE/mg. Compared to the control, the content of polysaccharide and total phenolic were increased by 1.42, 2.15-fold respectively.The concentration of the extract was 6 μg/mg, DPPH free radical scavenging capacity increased from 24.63% to 72.4%, reducing power increased from 0.11 to 0.38, ABTS+·radical scavenging capacity increased from 76.76% to 88.71%, FRAP from 39.08 μmol/L to 269.55 μmol/L. The principal component analysis and correlation analysis showed that the antioxidant index and polysaccharide, cordycepin and total phenolic had a significant correlation ( p < 0.01) . Thus, this study showed that fermented okara with enhanced functional ingredientsand antioxidant activities can be developed as food contained cordycepin and antioxidants.

     

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