Abstract: A graphene modified electrode was constructed by casting, and gold nanoparticles were reduced to graphene modified electrode surface.Electrochemical behavior of allura red at the gold nanoparticle/grapheme modified glassy carbon electrode was investigated.A new method was developed for the electrochemical analysis of allura red.The experimental results showed that a reversible redox peak was found on the gold nanoparticle-graphene modified glassy carbon electrode in the range of-0.8 to0.8 V in pH = 5.0 phosphate buffer solution. Gold nanoparticle/grapheme modified glassy carbon electrode showed a good catalytic performance for the electrochemical oxidation of allura red. The oxidation peak currents of allura red showed a linear relationship with their concentrations in the ranges of 8.00 × 10^-81.00 × 10^-5 mol/L.The detection limit was 8.00 × 10^-9 mol/L.The recovery rate was between 96.9% and 101.6%. The modified electrode has good selectivity and stability, and would be successfully applied to the detection of allura red in food samples.Therefore, this method could be used to determine the content of allura red in food.