水酶法从红花籽中提取油脂与水解蛋白的工艺优化

Process optimization of oil and hydrolyzed protein from safflower by hydrophobic enzyme

摘要: 以水酶法从红花籽中提取油脂和水解蛋白为目的,采用单因素和正交实验的方法,确定最佳的工艺条件:碱提温度为60 ℃,碱提时间为60 min,在水解蛋白酶Alcalase 2.4L、果胶酶和纤维素酶(1：1：1)共同作用下,酶解温度为55 ℃,酶解时间为3 h,料液比为1：5 (w/w),酶总添加量为4.5%,此时,红花籽游离油得率为58.21%,水解蛋白得率为41.32%。对于酶解后得到的乳状液选取最佳破乳方法为冷冻破乳,离心转速为8000 r/min,此时的破乳率为35.28%,总油得率为63.94%。

Abstract: The safflower oil and hydrolyzed protein were simultaneously extracted by aqueous enzymatic method. The optimum conditions were determined by single factor and orthogonal test. The optimum conditions were determined as follows:alkali temperature was 60℃, alkali time was 60 min, and hydrolyzed by protease (1:1:1), the enzymolysis temperature was 55℃, the enzymolysis time was 3 h, the ratio of material to liquid was 1:5 (w/w), the total enzymes was 4.5%. The yield of safflower seed was 58.21%, and the yield of hydrolyzed protein was 41.32%. The optimal demulsification method was selected for the emulsion obtained after enzymolysis. The demulsification method was determined as freezing and demulsification. The centrifugal speed was 8000 r/min. The breaking rate was 35.28% and the total oil yield was 63.94%.