Abstract: Objective:The objective of this work was to investigate the effect of Boletus edulis polysaccharide on the liver injury in type 2 diabetes mellitus (T2DM) rats. Methods:The rats were fed with high-fat high-sugar fodder and then injected with streptozocin (STZ) to induce T2DM rat model. Then the T2DM rats were divided randomly into model group, Bolete polysaccharide dose groups (200, 400 mg/kg·bw) and metformin group (200 mg/kg·bw), the normal rats were used as blank control, the normal and model group rats were fed with normal saline. The drugs were infused to stomach of every group of rats and once a day for 4 weeks. Then the levels of blood glucose (GLU) were detected, the liver indexs were calculated;the contents of triglyceride (TG), the activities of Glutamic-pyruvic Transaminase (ALT) and Glutamic oxalacetic Transaminase (AST) in serum were measured;the activities of catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) and the contents of glutathione (GSH), malonaldehyde (MDA) in the liver were detected. Results:Compared with the model group, three drug groups showed obviously lower contents of GLU, TG and MDA, liver index, activities of ALT and AST;and higher activities of CAT, SOD and GSH-Px, contents of GSH, the difference was significant. Conclusion:Boletus edulis polysaccharide can observably relieve the liver injury in T2DM rats.