响应面法优化脂肪酶水解红花籽油工艺

Optimization of Lipase Hydrolysis of Safflower Seed Oil by Response Surface Methodology

摘要: 以红花籽油为原料,采用不同脂肪酶水解以提高多不饱和脂肪酸水解率。研究酶添加量、酶解温度、pH、超声时间对水解率的影响,通过响应面实验结果表明:米曲霉脂肪酶酶加量300 U/g、温度39℃、pH7.0、酶解10 h为最佳工艺条件,此时酶解红花籽油的水解率最高,达到89.37%±0.19%。

Abstract: Using safflower seed oil as raw material,different lipase hydrolysis was used to improve poly unsaturated fatty acids. The effects of enzyme addition,temperature,pH and ultrasonic time on the hydrolysis rate were studied by single factor experiment. The optimum conditions were as follows:Aspergillus oryzae lipase dosage 300 u/g,temperature 39℃,pH7. 0,hydrolysis time 10 h,the hydrolysis rate of safflower seed oil was 89.37%±0.19%.