Abstract: The purpose of this study was to investigate the effects of resveratrol on body weight, feed intake, lipid metabolism, glucose metabolism and energy metabolism in obesity mice fed with high fat diet. Forty male C57BL/6J black mice of six-week-old were randomly divided into 4 groups, LFD, LFD+0.0276% (LR), HFD, HFD+0.04% (HR), fed 12 weeks after sampling. Results:Compared with the mice fed with low fat diet, the body weight of mice fed with high fat diet significantly increased (p<0.05), and the feed intake decreased significantly in the first 4 weeks (p<0.05). The activities of aspartate aminotransferase (AST) and glutamic-pyruvic transaminase (ALT) in serum increased significantly (p<0.05), and the activities of ALT and AST in the liver decreased significantly (p<0.05). The contents of lipid peroxides (LPO) and malondialdehyde (MDA) increased significantly (p<0.05), and the activities of hexokinase (HK), pyruvate kinase (PK), and succinate dehydrogenase (SDH) decreased significantly (p<0.05). In addition, the expression of MDH1, Sdha, PGC-1α mRNA in mice fed with high fat diet decreased significantly (p<0.05). Compared with the mice fed with high fat diet, the body weights of mice fed with high fat diet containing resveratrol decreased significantly (p<0.05), the feed intake increased significantly (p<0.05) and the activities of AST and ALT in serum decreased significantly (p<0.05), the activity of ALT in the liver was significantly increased (p<0.05), the contents of LPO and MDA were significantly lower (p<0.05), the activities of HK and PK were significantly increased (p<0.05). In addition, the expression of Hk2, Pkm, Cs, MDH1 and Sdha mRNA in high fat diet group fed with resveratrol was significantly increased (p<0.05). In summary, the addition of 40 mg/kg resveratrol to the diet can alleviate the damage caused by the high-fat diet to a certain extent and repair the lipid metabolism, glucose metabolism and mitochondrial energy metabolism.