微波辅助法制备南美白对虾甲壳素的工艺优化

Optimization of Preparation Process of Chitin from Penaeus Vannamei by Microwave Assisted Method

摘要: 以广西北部湾地区南美白对虾为原料,采用微波消解辅助法制备甲壳素,并利用单因素实验和正交试验对制备工艺进行了优化。结果表明:最佳脱蛋白工艺条件为NaOH浓度6%,微波功率240 W,微波时间7 min,料液比1:10 g/mL,最佳脱钙工艺条件为HCl浓度5%,微波功率160 W,微波时间3 min,料液比1:40 g/mL。在此条件制得的甲壳素脱蛋白率70.85%,脱钙率为91.26%,甲壳素得率为26.34%±0.24%,优于传统酸碱法甲壳素得率18.43%±0.14%。

Abstract: Chitin, from Penaeus vannamei in the Beibu Gulf of Guangxi, was prepared by microwave-assisted digestion. The preparation process was optimized by single factor experiment and orthogonal experiment.The results showed that the optimum deproteinized conditons were NaOH concentration 6%, microwave power 240 W, microwave time 7 min, material solution at 1:10 g/mL. and the optimum decalcification conditions were HCl concentration 5%, microwave power 160 W, microwave time 3 min, liquid ratio 1:40 g/mL.Under this condition,, deprotein rate reached 70.85%, decalcification rate was 91.26%, and the yield of chitin was 26.34% ±0.24%, which was better than that of the traditional acid base method 18.43% ±0.14%.