Abstract: Total flavonoids were extracted by alcohol extraction and ultrasonic assisted method from Tussilago farfara L. to investigate the the anti-hyperlipidemic and anti-oxidative effects in hyperlipidemia mice.The results showed that the yield of total flavonoids from Tussilago farfara L. was about 7.18% under the conditions of extraction time 30 min, ethanol volume fraction 80%, liquid ratio 41:1 (mL:g) and ultrasonic temperature 58℃, the best extraction process of total flavonoids from Tussilago farfara L. was stable and feasible. Purified by SP-825 macroporous resin, the purity of total flavonoids of Colletotrichum was 63.2%.Tussilago farfara L.total flavonoids can effectively regulate the concentration of TC, TG, LDL-C, HDL-C, and lipid-lowering effect in dose dependence; can also reduce the content of MDA in liver tissue, increased the contents of GSH and T-SOD, shows that Tussilago farfara L. toal flavonoids can reduce blood lipid level in hyperlipidemia mice, and has a certain antioxidant effect.