Abstract: In this experiment, theaflavins, thearubigins and theabrownine were studied in order to study the effect and mechanism of the intestinal flora of rats to control the effect of obesity.The model was established by feeding high-fat diet to nutritional the obesity wistar rat model, at the same time consecutive for nine weeks lavage the theaflavins, thearubigins and theabrownine, followed by determination of each rat body weight, Lee's index, fat weight and blood lipid levels, and through high-throughput sequencing technologies, the intestinal contents of rat intestinal flora of bioinformatics analysis. The results showed that, compared with the control group, the theaflavins, thearubigins and theabrownine could inhibit the weight gain (p < 0.05) and Lee's index (p < 0.01) of the rats, reduce their fat weight (p < 0.01 or p < 0.05), reduce the TC, HDL-C、LDL-C、TG content and AI index in rats'serum (p < 0.05 or p < 0.01), and inhibit the obesity effect of rats. Theaflavins, thearubigins and theabrownine could inhibit the growth of intestinal flora abundance and diversity of intestinal contents in rats. From the structure of the intestinal flora, it also had the ability to reduce the relative abundance of the Firmicutes, and increase the relative abundance of the Bacteroidetes, and the ratio of F/B was significantly reduced (p < 0.05). In addition, the theaflavins, thearubigins and theabrownine were also able to reduce the relative abundance of Ruminococcus in various groups of rats, and the relative abundance of Lactobacillus, Akkermansia and Lachnospiraceae had been recovered.That was, theaflavins, thearubigins and theabrownine had good regulation effect on high-fat diet rats.