降解玉米赤霉烯酮菌株的鉴定及其发酵条件优化

Identification of Degradation Zearalenone Strain and Its Fermentation Conditions Optimization

摘要: 从土壤样品中得到一株能降解玉米赤霉烯酮(zearalenone,ZEN)的菌株ZENL09,对该菌株的形态特征、生化理化特性和16r sRNA进行分析鉴定,并通过单因素实验得出ZENL09的较优的发酵条件。结果表明:ZENL09为枯草芽孢杆菌(Bacillus subtilis),其较优的发酵条件为:发酵时间24 h,发酵温度33℃,摇床转速200 r/min,装液量25 mL/250 mL,接种量3%,在此条件下,ZENL09发酵上清液对ZEN的降解率达到了96%。

Abstract: A strain ZENL09 capable of degrading zearalenone(ZEN)was obtained from soil samples. The morphological characteristics,biochemical and physiological characteristics of the strain and 16S ribosomal RNA gene sequence were analyzed and identified,and the superior fermentation conditions of ZENL09 were obtained by single factor experiments.The results showed that ZENL09 was Bacillus subtilis,the optimum fermentation conditions were:Fermentation time 24 h,fermentation temperature 33℃,shaking speed 200 r/min,liquid volume 25 mL/250 mL,and inoculation volume 3%.Under the optimal fermentation conditions,the ZEN degradation rate of ZENL09 fermentation supernatant reached 96%.