Abstract: In order to solve the problem of low yield caused by insufficient oxygen supply during the aerobic fermentation of poly-γ-glutamic acid (γ-PGA). The effects of different oxygen-vectors on γ-PGA fermentation was studied. The addition amount and time of the most effective oxygen-vector were optimized as well. The results showed that n-hexadecane was the most effective oxygen-vector. The optimal addition condition was 0.5% (V:V) and the adding time was 0 h. After 48 h fermentation,the yield of γ-PGA was increased to (18.62±0.84) mg/mL,which increased more than 30% compared with the control group. This study provided a theoretical basis for increasing the yield of γ-PGA by addition of oxygen-vectors to increase dissolved oxygen.