摘要:
目的:探究灵芝55%醇提物对高脂膳食大鼠脂质代谢及粪便菌群的影响。方法:将24只大鼠随机分为三组,分别为空白组(基础饲料+蒸馏水,NFD)、模型组(高脂饲料+蒸馏水,HFD)和干预组(高脂饲料+150 mg/kg·d灵芝55%醇提物,GLE),每次灌胃2 mL,连续喂养8周,测量血清、肝脏的生化指标和肝脏中与脂质代谢相关mRNA的表达量,并收集第4周和第8周粪便进行菌群结构分析。结果:与模型组相比,灵芝提取物显著(p<0.05)降低血清和肝脏中的甘油三酯(TG)、胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)含量,并显著(p<0.05)提高肝脏中高密度载脂蛋白固醇(HLD-C)含量。RT-qPCR分析显示灵芝提取物极显著(p<0.01)提高过氧化物酶体增殖剂激活受体α(PPARα)的转录水平,同时抑制固醇调节元件结合蛋白(SREBP-1C)和载脂蛋白B(ApoB)的转录水平。此外,灵芝提取物能有效改善粪便菌群结构,尤其增加考拉杆菌属(Phascolsrctobacterium)、粪球菌属(Coprococcus)、萨特菌属(Sutterella)和脱硫化弧菌属(Desulfovibrio)的丰富度;并显著降低厌氧棍状菌属(Anaerotruncus)、多尔氏菌属(Dorea)、链球菌属(Streptococcus)、乳酸杆菌属(Lactobacillus)和帕拉普氏菌属(Paraprevotella)的丰富度。结论:高脂饮食诱导Wistar大鼠脂质代谢紊乱和菌群结构失调,灵芝提取物干预可改善高脂膳食大鼠脂质代谢紊乱及其肠道菌群失调。
Abstract:
Objective:To study the influence of Ganoderma lucidum extracts(GLE)intervention on gut microbiota and lipid metabolism in rat by hight-fat diet. Methods:Twenty four rats were randomly divided into three groups,including control group(standard normal diet+distilled water),model group(high-fat diet+distilled water,HFD)and GLE group(high-fat diet+150 mg/kg·d GLE,GLE). All treatments were conducted with 2 mL/d by oral administration for 8-week continuous period. After 8 weeks,serum and liver markers and related mRNA expression levels were measured,and the feces for fourth weeks and eighth weeks were collected. Results:Compared with HFD group,oral administration of GLE markedly alleviated the dyslipidemia through decreasing the levels of serum and liver total triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C)(p<0.05),and increasing high-density lipoprotein cholesterol(HDL-C)in liver(p<0.05). In addition,GLE treatment resulted in reductions in mRNA levels of SREBP-1C and ApoB,but elevations in PPARα gene expression(p<0.01). Furthermore,GLE supplementation altered the composition of gut microbiota,especially increased the relative abundance of Phascolsrctobacterium,Coprococcus,Sutterella and Desulfovibrio,and reduced the relative abundance of Anaerotruncus,Dorea,Streptococcus,Lactobacillus and Paraprevotella. Conclusion:GLE could improve the lipid metabolism disorder and intestinal flora imbalance in Wistar rats by high-fat diet.
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