通过测定六株人源益生菌的自凝集及疏水性能力,并以人结肠腺癌细胞系Caco-2和HT-29作为体外细胞黏附模型评价其黏附能力,最后探究生长阶段、菌体浓度和孵育时间对发酵乳杆菌CECT5716黏附能力的影响。结果表明嗜酸乳杆菌F-1的自凝集率最高,为69.92%;疏水性最强的是鼠李糖乳杆菌MP-108,达到90.10%;乳双歧杆菌AD011对Caco-2和HT-29的黏附性均为最强,分别为(42.80±0.68)、(47.01±0.20) CFU/cell;不同益生菌的自凝集能力、疏水性及黏附能力均存在菌株特异性,其自凝集能力、疏水性及黏附能力间无明显相关性。此外,发酵乳杆菌CECT5716黏附能力随菌体浓度增大而显著(p<0.05)增加,当菌体浓度达108 CFU/mL以上时黏附能力不再显著提高;随着共孵育的时间延长,其黏附能力也增强,在共孵育2 h后不再提升;且菌体生长稳定期时表现出的黏附能力最强,达到(3.61±0.20) CFU/cell。所考察的益生菌大部分都具有较强的潜在黏附能力,其中以乳双歧杆菌AD011最强。这些益生菌具有可在人体肠道定殖生长的潜在益生功能,在食品和药品等行业中具有良好的应用前景。
The auto-aggregation and hydrophobicity of six human origin probiotics were determined,and their adhesion ability in vitro cell adhesion models was evaluated using the human colon cancer cell lines Caco-2 and HT-29. Finally,the effects of growth stage,cell concentration and incubation time on the adhesion of Lactobacillus fermentum CECT5716 were investigated. The results showed that the auto-aggregation rate of Lactobacillus acidophilus F-1 was the highest(69.92%). And the most hydrophobic was Lactobacillus rhamnosus MP-108(90.10%). Bifidobacterium lactis AD011 had the strongest adhesion to Caco-2 and HT-29,(42.80±0.68),(47.01±0.20) CFU/cell,respectively. There were strain specificity in the auto-aggregation ability,hydrophobicity and adhesion ability among different probiotics,but there was no significant correlation among the auto-aggregation ability,hydrophobicity and adhesion ability. In addition,the adhesion ability of L. fermentum CECT5716 improved significantly(p<0.05)with the increase of the concentration of the bacteria,but the adhesion ability no longer increased obviously when the concentration of the bacteria reached 108 CFU/mL or higher than it. As the time of co-incubation prolonged,the adhesion ability was also enhanced,but it had no longer increased after 2 h of co-incubation;and the cell exhibited the strongest adhesion ability in the stationary phase,reaching(3.61±0.20)CFU/cell. Most of the probiotics examined had strong potential adhesion,of which B. lactis AD011 was the strongest. These probiotics have potential probiotic properties of colonization and growth in human intestinal tract,and have good application prospects in food and pharmaceutical industries.