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中国精品科技期刊2020
燕傲蕾, 肖清臣, 周奎, 王玉民. 苔干酶促褐变归因分析[J]. 食品工业科技, 2019, 40(11): 102-107. DOI: 10.13386/j.issn1002-0306.2019.11.018
引用本文: 燕傲蕾, 肖清臣, 周奎, 王玉民. 苔干酶促褐变归因分析[J]. 食品工业科技, 2019, 40(11): 102-107. DOI: 10.13386/j.issn1002-0306.2019.11.018
YAN Ao-lei, XIAO Qing-chen, ZHOU Kui, WANG Yu-min. Attribution Analysis of Lactuca sativa Var. Angustata Enzymatic Browning[J]. Science and Technology of Food Industry, 2019, 40(11): 102-107. DOI: 10.13386/j.issn1002-0306.2019.11.018
Citation: YAN Ao-lei, XIAO Qing-chen, ZHOU Kui, WANG Yu-min. Attribution Analysis of Lactuca sativa Var. Angustata Enzymatic Browning[J]. Science and Technology of Food Industry, 2019, 40(11): 102-107. DOI: 10.13386/j.issn1002-0306.2019.11.018

苔干酶促褐变归因分析

Attribution Analysis of Lactuca sativa Var. Angustata Enzymatic Browning

  • 摘要: 为分析苔干酶促褐变的主要影响因素,以"涡青一号"苔干为研究对象,研究了春苔去皮后,苔干三个不同部位(苔头、苔茎、苔根)的褐变度、总酚含量、多酚氧化酶(PPO)活性和过氧化物酶(POD)活性在六个时间点(0、4、8、20、30、48 h)中的大小,褐变度、总酚含量、PPO活性和POD活性在各时间段内的增长速度,以及苔干不同部位、不同时间点的褐变度分别与总酚含量、PPO活性、POD活性的相关性。结果表明:苔干去皮8~30 h 内褐变度显著提高(p<0.05),30 h后,苔头的褐变情况明显高于苔茎和苔根,是苔干褐变控制的重点部位;总酚含量随时间增加先上升后下降,在20 h达到峰值;PPO、POD活性均随着时间增加而升高,但PPO、POD活性增速分别在8~20 h、20~30 h达到最高水平。在相关性方面,PPO、POD活性与苔头、苔茎和苔根的褐变度均极显著相关(p<0.01),总酚含量与苔干去皮0 h内、30 h时的褐变度显著相关(p<0.05),PPO、POD活性与苔干去皮48 h时的褐变度极显著相关(p<0.01);PPO和POD酶活性是影响苔干酶促褐变的关键因素。

     

    Abstract: To analyze the main contributory factors to enzymatic browning of Lactuca sativa var. angustata,browning degree,total phenol content,polyphenol oxidase(PPO)activity,peroxidase(POD)activity of Lactuca sativa var. angustata head,stem and root in 0,4,8,20,30,48 h and their increasing rates in each time period,the correlation coefficients between browning degree and total phenol content,PPO activity,POD activity in different parts and in different times were respectively investigated with peeled spring moss. The results showed that the browning degree of Lactuca sativa var. angustata improved significantly between 8~30 h(p<0.05),the browning degree of the head was much higher than the stem and root of Lactuca sativa var. Angustata,so the head was the important part to control the browning. Total phenol content increased at first and then decreased with time,and it achieved the peak value at 20 h;The activity of PPO and POD increased gradually with the time progress,while the increasing rate of PPO and POD activity reached highest level respectively in 8~20 h and 20~30 h. The correlation coefficients showed that both PPO activity and POD activity had extra significant correlation with browning degree of Lactuca sativa var. angustata head,stem and root(p<0.01);Total phenol content had significant correlation with browning degree at 0,30 h after peeled(p<0.05);PPO activity and POD activity had extra significant correlation with browning degree at 48(p<0.01);PPO activity and POD activity were the key factors of Lactuca sativa Var. Angustata enzymatic browning.

     

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