Abstract: Lactobacillus plantarum ZXH-1304S was induced with MRS medium contain uric acid and creatinine in vitro. The ability of strain on degradation of uric acid and creatinine were detected and the ability of tolerance of acid and cholate was investigated. Hyperuricemia rat model was established using oteracil potassium and adenine gavage. And the effect of Lactobacillus plantarum ZXH-1304S on uric acid and creatinine of rat model was investigated. The experiment results in vitro showed that the strain could degrade creatinine and uric acid obviously,and had ability of tolerance acid and cholate. In vivo study indicated that this strain could reduce creatinine and uric acid in rat model extremely obviously(p<0.01). Lactobacillus plantarum ZXH-1304S had ability of degrade creatinine and uric acid,being expected to be a substitution therapy or complementary therapy of hyperuricemia.