摘要:
目的:研究裂叶荨麻70%乙醇提取物对糖尿病小鼠肝脏损伤的保护作用。方法:采用腹腔注射链脲佐菌素(STZ)构建Ⅱ型糖尿病小鼠模型。将糖尿病小鼠随机分为模型组、裂叶荨麻70%乙醇提取物低、中、高剂量组(100、200、400 mg/kg),以健康ICR小鼠作为空白组,除空白组、模型组灌胃生理盐水外,各药物组灌胃不同剂量荨麻提取物,连续30 d,计算各组小鼠肝脏指数;HE染色观察小鼠肝脏组织病理变化,测定肝脏丙二醛(MDA)及蛋白羰基化(PCO)含量,测定超氧化物歧化酶(SOD)及过氧化氢酶(CAT)活力。结果:模型组小鼠肝脏指数增大,肝脏组织出现明显病理损伤,MDA、PCO含量明显增加(p<0.01),SOD、CAT活性明显下降(p<0.01);与模型组相比,裂叶荨麻70%乙醇提取物能不同程度降低肝脏指数(p<0.01),减少MDA、PCO含量(p<0.05),提高SOD、CAT活性(p<0.01),改善肝脏氧化损伤。结论:裂叶荨麻70%乙醇提取物对糖尿病小鼠的肝脏具有保护作用,其保护机制可能与抗氧化作用有关。
关键词:
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裂叶荨麻 /
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糖尿病 /
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肝脏 /
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抗氧化
Abstract:
Objective:To investigate the protective effect of 70% ethanol extract of Urtica fissa on the liver injury in diabetic mice. Methods:Type 2 diabetes mellitus model was induced by intraperitoneal injection of streptozotocin in mice. The diabetic mice were randomly divided into 4 groups:model group,70% ethanol extract of Urtica fissa low groups(100 mg/kg),medium groups(200 mg/kg),high groups(400 mg/kg),and the normal mice were the control group. control group and model group were administrated with normal saline,and five groups mice were continuously administered for 30 days. The liver index of mice was calculated,and the liver pathological change were observd by HE stain. The contents of malondialdehyde(MDA)and protein carbonyl of(PCO),the activities of superoxide dismutase(SOD)and catalase(CAT)in the liver were detected. Results:Compared with the control group,the liver index of mice in model groups increased,the liver tissue was obviously injuried,the contents of MDA and PCO were significantly increased(p<0.01),the activities of SOD and CAT were reduced(p<0.01). Compared with the model group,70% ethanol extract of Urtica fissa groups showed obviously decreased the liver index of mice(p<0.01),the contents of MDA and PCO were reduced(p<0.05),the activities of SOD and CAT were increased(p<0.01). Conclusion:70% ethanol extract of Urtica fissa can observably protect the liver of diabetic mice,and the mechanism may be related to antioxidation effect.
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