本文优化了山楂多糖的微波辅助提取工艺,并对其降血糖降血脂活性进行了研究。在考察固液比、提取温度、微波功率、提取时间对山楂多糖提取量影响的基础上,采用Box-Behnken响应面法对提取工艺进行优化。通过测定初步纯化的山楂粗多糖对α-葡萄糖苷酶、胰脂肪酶抑制率和DPPH·清除率,评价山楂多糖的降血糖、降血脂活性。结果表明,山楂多糖的最佳提取工艺条件为:提取温度63 ℃,微波功率500 W,提取时间7 min,在此条件下山楂多糖提取量为(147.10±0.32) mg/g。山楂粗多糖对α-葡萄糖苷酶、胰脂肪酶抑制率和DPPH·清除率的IC50分别为(99.22±0.89) μg/mL、(22.50±0.79) mg/mL、(185.80±0.64) μg/mL,表明山楂粗多糖具有一定的降血糖、降血脂作用。
The microwave-assisted extraction technology of hawthorn polysaccharide was optimized and its hypoglycemic and lipid-lowering activities were studied in this paper. On the basis of investigating the effect of solid to liquid ratio, extraction temperature, microwave power and extraction time on the yield of hawthorn polysaccharide, the Box-Behnken response surface method was used to optimize the extraction process. The effects of hawthorn crude polysaccharide on α-glucosidase, pancreatic lipase inhibition rate and DPPH·clearance rate were determined to evaluate the hypoglycemic and lipid lowering activities of hawthorn crude polysaccharides. The results showed that, the optimum extraction conditions of hawthorn polysaccharide were as follows:the extraction temperature was 63℃, the microwave power was 500 W, the extraction time was 7 min, and the extraction rate amount of hawthorn polysaccharide was (147.10±0.32) mg/g under these conditions. The IC50 of hawthorn polysaccharide on alpha glucosidase, pancreatic lipase, cholesterol esterase inhibition rate and DPPH·clearance rate were (99.22±0.89) μg/mL, (22.50±0.79) mg/mL and (185.80±0.64) μg/mL, respectively, which showed that hawthorn polysaccharide had certain hypoglycemic and blood lipid lowering effects.