响应面优化二阶段法粗糙脉孢菌发酵产番茄红素工艺

Fermentation Conditions Optimization of a Two-stage Culture for Enhancing Production of Lycopene by Neurospora crassa Using Response Surface Methodology

摘要: 本文研究了二阶段培养法对粗糙脉孢菌发酵产番茄红素的影响,通过Box-Behnken实验设计和响应面优化二阶段培养粗糙脉孢菌发酵产番茄红素的工艺条件。结果表明,最佳发酵工艺条件为:摇瓶培养时间19 h,静止培养时间87 h,接种量5%,培养基浓度35 g/L,温度30℃,摇瓶速率100 r/min。在此条件下,相对传统摇床培养法,菌体干质量减少了23.54%,番茄红素的产量提高了102.54%。

Abstract: The effect of two-stage culture method(by combing shake culture with static culture)on lycopene production was studied in this study. Through Box-behnken experimental design and response surface methodology,the optimum conditions were shake culture 19 h,static culture 87 h,the concentration of medium 35 g/L,inoculum size of 5%,culture temperature 30℃ and bottle shaking rate 100 r/min. Under this conditions,the dry cell weight decreased by 23.54% and lycopene production increased by 102.54% compared with conventional shake culture.