响应面法优化圆弧青霉(CICC-4022)产右旋糖酐酶的培养条件

Optimization of Fermentation Conditions for Dextranase Produced by Penicillium cyclopium(CICC-4022)by Response Surface Methodology

摘要: 为了提高圆弧青霉菌(CICC-4022)发酵合成右旋糖酐酶的产量,对发酵培养条件进行优化。采用单因素实验,研究装液量、发酵温度、培养基初始pH、摇床转速对发酵产酶的影响;在单因素实验的基础上,采用响应面实验对圆弧青霉产酶培养条件进行优化,确定培养条件的交互作用及最优组合。结果表明,最适培养条件为:装液量为60 mL/250 mL、发酵温度为30℃、培养基初始pH为6.0、最适转速为160 r/min,此时右旋糖酐酶酶活力达到(53.68±0.12)U/mL,比优化前提高30.10%±0.08%。

Abstract: In order to improve the yield of dextranase biosynthesis produced by Penicillium cyclopium(CICC-4022),the cultivation conditions were optimized. The effects of liquid volume,fermentation temperature,initial pH and shaking speed on dextranase production were studied in the fermentation process by single factor experiments. On the basis of single factor experiments,the interaction and optimal combination of fermentation conditions were determined by response surface methodology. The results showed that the optimum conditions were determined as follows:Flask volume 60 mL/250 mL,fermentation temperature 30℃,initial pH of culture medium 6.0,and the shaking speed 160 r/min,the dextranase activity reached(53.68±0.12) U/mL,which was 30.10%±0.08% higher than before optimization.