Abstract: A method for determination of four eugenol derivatives(eugenol,iso-eugenol,methyl eugenol and methyl iso-eugenol)in aquatic products by gas chromatography-mass spectrometry with solid-phase extraction(SPE-GC/MS)was established. The samples were extracted with N-hexane-acetone mixture(3:1,v/v). The extract was cleaned up with silica solid phase extraction column after being purified by PSA and concentrated. The target compounds were detected by GC-MS with SIM mode. The external standard method was applied for quantitative analysis. As a result,The calibration curves of four eugenol derivatives showed good linearity in the range of 5.0~200 ng/mL with correlation coefficients(r)was over 0.998. The limits of detection(LOD)and the limits of qualification(LOQ)were 3.0,10.0 μg/kg,respectively. When the spiked concentrations were 0.01,0.05,0.20 mg/kg,the average recoveries of four target compounds were of 85.7%~102.5% with the relative standard deviations(RSD)of 3.3%~9.8%.This method was suitable for determination of four eugenol derivatives in aquatic products qualitatively and quantitatively with satisfactory purification effect and sensitivity.