Abstract: Objective:To investigate the inhibitory effect of Lactobacillus plantarum KLDS1.0344 and KLDS1.0386 on obesity formation in mice. Methods:The experimental mice were divided into blank group,model group,intervention group and drug group. The blank group was fed D12450B control feed,the other three groups were fed D12492 high fat diet,the blank group and the model group were intragastrically sterilized with PBS buffer solution and intervention group(KLDS1.0344 group,KLDS1.0386 group,mixed group)and drug group were fed 0.2 mL gastric suspension(mixed group according to 1:1 ratio,bacterial concentration 5×10⁸ CFU/mL),orlistat(10 mg/kg),respectively for 8 weeks,the body weight,Lees coefficient,body fat percentage,blood lipids,liver triglycerides,liver HE slices and other related biochemical indicators were determined. Results:After 8 weeks,the weight of the model group was higher than that of the blank group by 27%,and the model was successfully established. Compared with the weight gain of the model group of 10.49 g,the weight gain of the intervention group was significantly decreased by 5.36 g(P<0.05),and the Lees coefficient was 3.12%. Body fat percentage was 2.33%,blood lipids(TC:4.23 mmol/L,TG:0.80 mmol/L)and liver triglyceride content(0.28 mmol/g prot)were significantly decreased(P<0.05);models were observed from liver sections. Significant steatosis occurred in the liver of the group,and significant remission in the intervention group(P<0.05). Conclusion:This combined strain can be used as a potential probiotic preparation to inhibit the formation of high fat-induced obesity.