Abstract: Through field experiments,7 kinds of plant growth regulators with different concentrations were sprayed on"red globe"grapes,and the residue of growth regulators in grape fruits and cobs were detected by UPLC-MS/MS method,and the residual digestion dynamics were obtained. The results indicated that the degradation rate of plant growth regulators with different concentrations was faster within 30 d after spraying potions. The residual amount of 6 kinds of growth regulators in cob was higher than that in fruit except cycocel. When the grape berries were ripe,the residue of grape berries and spike-stalk decreased to below the detection limit(mepiquat chloride<0.01 μg/kg,cycocel<0.01 μg/kg,6-BA<0.002 μg/kg,2-diethylaminoethyl hexanoate<0.02 μg/kg,thidiazuron<0.005 μg/kg,gibberellin<0.20 μg/kg,forchlorfenuron<0.01 μg/kg). The content of indoleacetic acid and abscisic acid could be detected by this method,their content did not change with the spraying of exogenous growth regulator. The UPLC-MS/MS method could accurately and quantitatively detect the content of plant growth regulators in grapes. The 7 kinds of plant growth regulators which sprayed to regulate the yield or quality of grapes in this experiment did not affect the edible safety of grapes in a certain concentration range.