采用响应面法优化超声辅助提取代代花总黄酮的工艺,并研究代代花总黄酮的抗氧化活性。本实验首先通过单因素实验,考察乙醇浓度、料液比、提取温度及时间对总黄酮得率的影响,然后采用四因素三水平响应面试验设计优化代代花总黄酮的最佳提取工艺,同时通过DPPH·和·OH的清除实验对代代花总黄酮的抗氧化活性进行评估。结果表明,最佳提取工艺条件是乙醇浓度60%、液料比20:1 mL/g、提取时间35 min、提取温度为60℃,该条件下代代花总黄酮的得率为2.62%,该值与预测值2.66%高度相符;其对DPPH·和·OH均有较强的清除作用,IC50值分别为0.385和0.255 mg/mL。通过响应面法优化的代代花总黄酮超声辅助提取工艺稳定可行,且代代花总黄酮提取物具有较强的抗氧化活性。
To optimize the ultrasonic-assisted extraction technology of total flavonoids from Citrus aurantium L. var daidai by response surface method and study its antioxidant activity. Firstly,the effects of ethanol concentration,solid-liquid ratio,extraction temperature and time on the extraction yield of total flavonoids by single factor experiment,then the extraction process was optimized by four-factor and three-level design of surface response analysis,at the same time,the antioxidant activity of flavonoids from Citrus aurantium L. var daidai was studied by assessing the DPPH· and ·OH scavenging activities. Results showed that the optimum process conditions for the extraction total flavonoids were as follows:ethanol concentration 60%,the ratio of liquid to solid 20:1 mL/g,extraction time 35 min,extraction temperature was 60 ℃,the extraction yield of total flavonoids from Citrus aurantium L. var daidai was 2.62% under such conditions,which was highly consistent with the predicted value of 2.66%;and the IC50 values of DPPH· and ·OH scavenging activities were 0.385 and 0.255 mg/mL,respectively. The extraction process optimized by response surface method was stable and feasible,and the total flavonoids from Citrus aurantium L. var daidai had strong antioxidant activity.