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中国精品科技期刊2020
王娜娜, 李柏良, 李娜, 史佳璐, 宋月, 霍贵成, 李艾黎. 植物乳杆菌KLDS1.0386联合色氨酸对溃疡性结肠炎的影响[J]. 食品工业科技, 2020, 41(12): 296-301. DOI: 10.13386/j.issn1002-0306.2020.12.049
引用本文: 王娜娜, 李柏良, 李娜, 史佳璐, 宋月, 霍贵成, 李艾黎. 植物乳杆菌KLDS1.0386联合色氨酸对溃疡性结肠炎的影响[J]. 食品工业科技, 2020, 41(12): 296-301. DOI: 10.13386/j.issn1002-0306.2020.12.049
WANG Na-na, LI Bai-liang, LI Na, SHI Jia-lu, SONG Yue, HUO Gui-cheng, LI Ai-li. Effect of Lactobacillus plantarum KLDS 1.0386 Combined with Degrading Tryptophan on Ulcerative Colitis[J]. Science and Technology of Food Industry, 2020, 41(12): 296-301. DOI: 10.13386/j.issn1002-0306.2020.12.049
Citation: WANG Na-na, LI Bai-liang, LI Na, SHI Jia-lu, SONG Yue, HUO Gui-cheng, LI Ai-li. Effect of Lactobacillus plantarum KLDS 1.0386 Combined with Degrading Tryptophan on Ulcerative Colitis[J]. Science and Technology of Food Industry, 2020, 41(12): 296-301. DOI: 10.13386/j.issn1002-0306.2020.12.049

植物乳杆菌KLDS1.0386联合色氨酸对溃疡性结肠炎的影响

Effect of Lactobacillus plantarum KLDS 1.0386 Combined with Degrading Tryptophan on Ulcerative Colitis

  • 摘要: 为探究色氨酸的降解对溃疡性结肠炎是否存在预防效果,本文通过高效液相色谱方法测定植物乳杆菌发酵液的色氨酸含量,利用实验室保藏的16株乳酸杆菌筛选出一株具有较高降解色氨酸能力的菌株。将75只8周龄C57BL/6N雄鼠分为5组:正常组、模型组、色氨酸组、1.0386组、1.0386+色氨酸组,分别以PBS、色氨酸、1.0386菌悬液以及1.0386和色氨酸混合物连续灌胃3周,在实验结束前最后一周,让小鼠饮用2.5%葡聚糖硫酸钠(DSS)7 d进行诱导溃疡性结肠炎小鼠模型,同时继续进行灌胃。造模期间,记录小鼠DAI;实验结束后,处死小鼠,测定结肠长度、器官重量以及结肠组织病理变化。结果表明,与正常组相比,DSS可显著降低小鼠体重,升高DAI指数,使结肠缩短并引起结肠组织损伤,上调血清中促炎因子,下调抑炎因子(P<0.05)。而植物乳杆菌KLDS1.0386联合色氨酸可显著改善DSS引起的体重下降、结肠缩短,并降低疾病活动指数和脾脏指数(P<0.05);此外,植物乳杆菌KLDS1.0386与色氨酸混合干预显著下调促炎因子IL-6含量,上调抑炎因子IL-10的含量(P<0.05)。因此,植物乳杆菌KLDS1.0386联合色氨酸可以有效改善溃疡性结肠炎小鼠的结肠病理损伤。

     

    Abstract: To study the preventive effect of Lactobacillus degrading tryptophan on ulcerative colitis, the content of tryptophan in fermentation broth was determined by high performance liquid chromatography (HPLC). 75 8-week-old C57BL/6N male mice were divided into five groups:Normal group, model group, tryptophan group, 1.0386 group, 1.0386+tryptophan group. PBS, tryptophan, 1.0386 bacterial suspension and the mixture of 1.0386 and tryptophan was continuously administered for 3 weeks. In the last week before the end of the experiment, the mice were given 2.5% dextran sulfate sodium (DSS) for 7 d to induce mice model of ulcerative colitis, and the mice were intragastrically administered. During the modeling period, DAI of mice were recorded.After the experiment, the mice were sacrificed, and colon length, organ weight, and pathological changes of colon tissue were measured. The results showed that compared with the normal group, DSS could significantly reduce the body weight of mice, increase DAI index, shorten colon and cause colon tissue damage, up-regulate proinflammatory factors in serum and down-regulate anti-inflammatory factors (P<0.05). What's more, Lactobacillus plantatus KLDS1.0386 combined with tryptophan could alleviate DSS-induced weight loss, colon shortening, disease activity index and spleen index (P<0.05). In addition, Lactobacillus plantatus KLDS1.0386 combined with tryptophan down-regulate the pro-inflammatory factor IL-6 and up-regulate the anti-inflammatory factor IL-10 (P<0.05). Therefore, Lactobacillus plantans KLDS1.0386 combined with tryptophan could improve the pathological damage of colitis mice.

     

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