采用响应面法优化结香花中总黄酮的提取工艺,评价其抗氧化活性。在单因素实验的基础上,以乙醇浓度、液料比、提取温度、提取时间为优化因素,总黄酮得率为评价指标,采用Box-Behnken法优化结香花总黄酮的提取工艺;利用DPPH和ABTS自由基清除实验检测结香花总黄酮的抗氧化能力。结果表明,结香花总黄酮的最佳提取工艺为乙醇浓度80%,液料比35:1,提取温度83℃,提取时间85 min,总黄酮得率22.76 mg·g-1。最佳工艺条件下得到的提取物具有一定的清除自由基能力,且与总黄酮含量呈明显的浓度依赖性,体外清除DPPH自由基和ABTS自由基的IC50值分别为0.75和0.98 mg·mL-1。
The response surface method was used to optimize the extraction process of total flavonoids from Edgeworthia chrysantha Lindl and its antioxidant activity was evaluated. Based on single factor experiments,taking ethanol concentration,liquid to material ratio,extraction temperature and extraction time as optimization factors,the total flavonoid yield was an evaluation index,and the Box-Behnken method was used to optimize the extraction process of total flavonoids. The DPPH and ABTS free radical scavenging experiments were used to detect the antioxidant capacity of total flavonoids. The results showed that the optimal conditions were determined to be 80% for ethanol concentration,35:1 for liquid to material ratio,83℃for extraction temperature,85 min for extraction time,the total flavonoids yield was 22.76 mg·g-1. The extract obtained under the optimal process conditions had a certain ability to scavenge free radicals,and had a significant concentration dependence on the total flavonoid content. The IC50 values of scavenging DPPH radicals and ABTS radicals in vitro were 0.75 and 0.98 mg·mL-1,respectively.