N-丙酰-唾液酸衍生物的化学酶法合成及在测定禽蛋中唾液酸含量的应用

王毛毛 张瑶瑶 姚宏亮 刘丽 JOSEF Voglmeir

王毛毛, 张瑶瑶, 姚宏亮, 刘丽, JOSEF Voglmeir. N-丙酰-唾液酸衍生物的化学酶法合成及在测定禽蛋中唾液酸含量的应用[J]. 食品工业科技, 2021, 42(1): 28-34,41. doi: 10.13386/j.issn1002-0306.2020010142
引用本文: 王毛毛, 张瑶瑶, 姚宏亮, 刘丽, JOSEF Voglmeir. N-丙酰-唾液酸衍生物的化学酶法合成及在测定禽蛋中唾液酸含量的应用[J]. 食品工业科技, 2021, 42(1): 28-34,41. doi: 10.13386/j.issn1002-0306.2020010142
WANG Mao-mao, ZHANG Yao-yao, YAO Hong-liang, LIU Li, JOSEF Voglmeir. Chemoenzymatic Synthesis of N-Propionyl-Neuraminic Acid Derivative and Its Application in the Determination of Sialic Acid Content in Poultry Eggs[J]. Science and Technology of Food Industry, 2021, 42(1): 28-34,41. doi: 10.13386/j.issn1002-0306.2020010142
Citation: WANG Mao-mao, ZHANG Yao-yao, YAO Hong-liang, LIU Li, JOSEF Voglmeir. Chemoenzymatic Synthesis of N-Propionyl-Neuraminic Acid Derivative and Its Application in the Determination of Sialic Acid Content in Poultry Eggs[J]. Science and Technology of Food Industry, 2021, 42(1): 28-34,41. doi: 10.13386/j.issn1002-0306.2020010142

N-丙酰-唾液酸衍生物的化学酶法合成及在测定禽蛋中唾液酸含量的应用

doi: 10.13386/j.issn1002-0306.2020010142
基金项目: 

国家自然科学基金(31471703,A0201300537,31671854);"外专百人计划"(JSB2014012)。

详细信息
    作者简介:

    王毛毛(1989-),女,硕士研究生,研究方向:糖组学与糖生物学,E-mail:2012108007@njau.edu.cn;张瑶瑶(1993-),女,博士研究生,研究方向:糖组学与糖生物学,E-mail:2018208001@njau.edu.cn。

    通讯作者:

    刘丽(1972-),女,博士,教授,研究方向:糖组学与糖生物学,E-mail:lichen.liu@njau.edu.cn

    JOSEF Voglmeir (1979-),男,博士,教授,研究方向:糖组学与糖生物学,E-mail:josef.voglmeir@njau.edu.cn。

  • 中图分类号: TS201.1

Chemoenzymatic Synthesis of N-Propionyl-Neuraminic Acid Derivative and Its Application in the Determination of Sialic Acid Content in Poultry Eggs

  • 摘要: 为实现禽类蛋黄和蛋清中N-乙酰神经氨酸(N-acetylneuraminic acid,Neu5Ac)的准确定量,消除禽蛋样品中分析物以外的物质产生的基质效应对分析结果造成的影响,本研究以甘露糖胺为底物采用化学酶法合成了非天然唾液酸衍生物5-溴-4-氯-3-吲哚-β-D-半乳糖苷-N-丙酰-唾液酸(5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside-N-propionyl-sialic acid,X-Gal-α-2,6-Neu5Prop),对该衍生物在酸性条件下的水解特性以及稳定性进行研究,并以该衍生物为内标测定了鹌鹑、鹅、珍珠鸡、鸵鸟、鸭、鸽子、火鸡等9种禽类蛋黄和蛋清中Neu5Ac的含量。禽蛋样品和内标物在酸性条件下处理,唾液酸糖缀合物被解离为游离的唾液酸后经荧光标记物邻苯二胺衍生,采用高效液相色谱-荧光检测器对样品进行分析。结果表明,相同浓度的X-Gal-α-2,6-Neu5Ac和X-Gal-α-2,6-Neu5Prop在2 mol/L的醋酸溶液80℃的水解条件下水解程度相当,90 min后能够完全转化为游离的唾液酸。Neu5Ac和Neu5Prop在10 h内仍保持相似的稳定性。蛋清中Neu5Ac的含量在不同物种之间表现出很大的差异。鹌鹑蛋清中Neu5Ac的含量最低(0.13 mg/g),而鸵鸟蛋清中的最高(2.20 mg/g),比鹌鹑蛋清Neu5Ac含量高出近17倍。该方法能够消除生物样品中的基质效应,实现对Neu5Ac的准确定量,且样品前处理简单。因此,适合常规食品中Neu5Ac的检测与分析。
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  • 收稿日期:  2020-01-15

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