表没食子儿茶素没食子酸酯稳定性研究

郝静 杨太琴 王晗 汪旭 倪娟

郝静, 杨太琴, 王晗, 汪旭, 倪娟. 表没食子儿茶素没食子酸酯稳定性研究[J]. 食品工业科技, 2021, 42(1): 48-53. doi: 10.13386/j.issn1002-0306.2020020035
引用本文: 郝静, 杨太琴, 王晗, 汪旭, 倪娟. 表没食子儿茶素没食子酸酯稳定性研究[J]. 食品工业科技, 2021, 42(1): 48-53. doi: 10.13386/j.issn1002-0306.2020020035
HAO Jing, YANG Tai-qin, WANG Han, WANG Xu, NI Juan. Study on the Stability of (-)-Epigallocatechin-3-gallate[J]. Science and Technology of Food Industry, 2021, 42(1): 48-53. doi: 10.13386/j.issn1002-0306.2020020035
Citation: HAO Jing, YANG Tai-qin, WANG Han, WANG Xu, NI Juan. Study on the Stability of (-)-Epigallocatechin-3-gallate[J]. Science and Technology of Food Industry, 2021, 42(1): 48-53. doi: 10.13386/j.issn1002-0306.2020020035

表没食子儿茶素没食子酸酯稳定性研究

doi: 10.13386/j.issn1002-0306.2020020035
基金项目: 

国家自然科学基金(31860301,31560307)。

详细信息
    作者简介:

    郝静(1996-),女,本科,研究方向:遗传学,E-mail:18487139512@163.com;杨太琴(1992-),女,硕士研究生,研究方向:人类遗传,E-mail:lanren789@126.com。

    通讯作者:

    倪娟(1982-),女,博士,副教授,研究方向:营养基因组学,E-mail:gt_gg30@163.com。

  • 中图分类号: TS255.1

Study on the Stability of (-)-Epigallocatechin-3-gallate

  • 摘要: 目的:解析表没食子儿茶素没食子酸酯((-)-epigallocatechin-3-gallate,EGCG)氧化聚合的环境条件,及其稳定性变化对体外培养细胞系氧化还原平衡的影响,为深入探讨EGCG在细胞培养体系及活体中的作用机制奠定基础。方法:在无细胞体系中,设置不同的环境条件,溶液环境(H2O、RPMI1640、DMEM)、温度(37和74℃)、EGCG浓度(0、20、40、80、320 μmol/L)、反应时间(3、6、12、24 h)、pH (5和9)等,通过检测OD578值变化评估EGCG在不同条件下氧化聚合的差异;分析EGCG氧化聚合变化对受试细胞内外H2O2水平的影响。结果:发现RPMI1640和DMEM培养液较H2O更利于EGCG的氧化聚合,高温高EGCG浓度及碱性条件促进EGCG氧化聚合,同时EGCG的氧化聚合随反应时间延长而加剧。在含有细胞的培养体系中EGCG也会发生自动氧化聚合并产生H2O2,导致细胞内外的H2O2浓度升高,但胞内H2O2浓度呈现先上升后下降的趋势。结论:溶液环境和pH变化是引起EGCG氧化聚合的关键因素,在细胞培养中20 μmol/L的EGCG可通过氧化聚合效应发挥更好的抗氧化活性。
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  • 收稿日期:  2020-02-05

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