硝酸盐促进环磷酸腺苷发酵合成的生理机制研究

李志刚 顾阳 陈宝峰 王宝石 张中华 常景玲

李志刚, 顾阳, 陈宝峰, 王宝石, 张中华, 常景玲. 硝酸盐促进环磷酸腺苷发酵合成的生理机制研究[J]. 食品工业科技, 2021, 42(1): 115-120,131. doi: 10.13386/j.issn1002-0306.2020030025
引用本文: 李志刚, 顾阳, 陈宝峰, 王宝石, 张中华, 常景玲. 硝酸盐促进环磷酸腺苷发酵合成的生理机制研究[J]. 食品工业科技, 2021, 42(1): 115-120,131. doi: 10.13386/j.issn1002-0306.2020030025
LI Zhi-gang, GU Yang, CHEN Bao-feng, WANG Bao-shi, ZHANG Zhong-hua, CHANG Jing-ling. Physiological Mechanism for Enhanced Cyclic Adenosine Monophosphate Biosynthesis by Sodium Nitrate in Arthrobacter sp. CCTCC M2013431[J]. Science and Technology of Food Industry, 2021, 42(1): 115-120,131. doi: 10.13386/j.issn1002-0306.2020030025
Citation: LI Zhi-gang, GU Yang, CHEN Bao-feng, WANG Bao-shi, ZHANG Zhong-hua, CHANG Jing-ling. Physiological Mechanism for Enhanced Cyclic Adenosine Monophosphate Biosynthesis by Sodium Nitrate in Arthrobacter sp. CCTCC M2013431[J]. Science and Technology of Food Industry, 2021, 42(1): 115-120,131. doi: 10.13386/j.issn1002-0306.2020030025

硝酸盐促进环磷酸腺苷发酵合成的生理机制研究

doi: 10.13386/j.issn1002-0306.2020030025
基金项目: 

河南省科技攻关项目(社会发展)(162102310419,182102310823);河南科技学院高层次人才科研启动项目(2015006)。

详细信息
    作者简介:

    李志刚(1984-),男,博士,讲师,研究方向:发酵过程优化与控制,E-mail:lizhigang25@126.com。

    通讯作者:

    常景玲(1963-),女,本科,教授,研究方向:发酵制药,E-mail:changjl001@126.com。

  • 中图分类号: TS201.3

Physiological Mechanism for Enhanced Cyclic Adenosine Monophosphate Biosynthesis by Sodium Nitrate in Arthrobacter sp. CCTCC M2013431

  • 摘要: 目的:阐明硝酸盐促进环磷酸腺苷发酵合成的生理机制。方法:首先通过摇瓶实验确定硝酸钠最适添加条件,并在发酵罐上进行添加硝酸钠的cAMP发酵实验,然后对发酵过程动力学数据、硝酸盐代谢、还原力水平、关键酶活性、氨基酸水平和能量代谢进行分析。结果:确定在发酵24 h添加3 g/L-broth硝酸钠为最适条件,cAMP发酵产量和得率分别达到5.02 g/L和0.097 g/g,比对照批次分别提高了22.7%和29.8%,发酵性能得到明显提升。硝酸盐利用过程消耗了大量NADPH,缓解了对6-磷酸葡萄糖脱氢酶的抑制作用,同时糖酵解途径受到抑制而磷酸戊糖途径和三羧酸循环中关键酶活性明显提高,更多碳流分配到产物合成途径。此外,胞内前体氨基酸水平、NADH/NAD+和ATP/AMP均得到明显提高,为cAMP发酵合成提供了物质和能量基础。结论:硝酸盐利用过程消耗了大量的还原力,改变了代谢流分配情况,提高了胞内氨基酸水平和ATP合成,进而促进cAMP的发酵合成。硝酸盐作用机制的阐明,为提高核苷酸类发酵产品的生产水平提供了参考。
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  • 收稿日期:  2020-03-03

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