Antioxidant Activity in Vitro and Vivo of Polysaccharide from Paulownia fortunei Flower
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摘要: 以泡桐花为原料水提醇沉法制备多糖，测定泡桐花多糖的体外、体内抗氧化活性。体外抗氧化测定结果显示，泡桐花多糖对超氧阴离子、羟基自由基清除以及抑制H2O2溶血有较好的效果，呈剂量依赖性；质量浓度0.5 mg/mL时，对超氧阴离子和羟基自由基清除率分别达到54.36%，74.62%，超过半数效应50%；质量浓度2 mg/mL时，抗H2O2氧化溶血率超过阳性对照组Vc（1 mg/mL）。体内抗氧化测定结果显示，泡桐花多糖低、中、高（日剂量5、10、20 mg/mL，0.2 mL）小鼠灌胃28 d对体质量无影响；血清和心脏、肝脏、肾脏、回肠脏器超氧化物歧化酶（superoxide dismutase，SOD）、还原型谷胱甘肽（glutathione，GSH）、丙二醛（malondialdehyde，MDA）含量和总抗氧化能力（total antioxidant capacity, T-AOC）检测表明，一空白组相比，不同剂量的泡桐花多糖能够增加或显著增加SOD、GSH含量和T-AOC水平（P<0.05），同时降低MDA含量，并存在一定的量效关系。研究表明泡桐花多糖具有良好的抗氧化活性，可作为天然抗氧化剂应用于食药工业。Abstract: This study aimed at evaluating the antioxidant activity in vitro and vivo of polysaccharide prepared from Paulownia fortunei flower by water extract-alcohol precipitation. In vitro, results showed that Paulownia fortunei flower polysaccharide had a dose-dependent and good effect on scavenging superoxide anion, hydroxyl radical and inhibiting H2O2 hemolysis. When the concentration of polysaccharide controlled at 0.5 mg/mL, the scavenging rates of superoxide anion and hydroxyl radical were 54.36% and 74.62% respectively, with more than half effect of 50%. When at 2 mg/mL, the anti-H2O2 oxidation hemolysis rate was higher than that of the positive control Vc group (1 mg/mL). In vivo, after the experimental mice were administered to oral gavage with Paulownia flower polysaccharide at low, medium and high dosages (5, 10 and 20 mg/mL, 0.2 mL) for 28 consecutive days. The result showed that Paulownia fortunei flower polysaccharide had no effect on weight of mice; different doses of polysaccharide could enhance or siginificantly enhance the content of superoxide dismutase (SOD), glutathione (GSH), total antioxidant capacity (T-AOC) remarkably in serum, heart, liver, kidney, ileum (P<0.05) when compared with that of the blank group, and reduce the malondialdehyde (MDA) content dramatically with a certain concentration-response relationship. The results indicate that Paulownia fortunei flower polysaccharide had perfect antioxidant activity and could be used as a natural antioxidant in food and medicine industry.
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Key words:
- Paulownia fortune flower polysaccharide /
- in vitro /
- in vivo /
- antioxidant
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图 2 泡桐花多糖对小鼠血清和组织器官GSH的影响（n = 4）
Figure 2. Effect of Paulownia fortune flower polysaccharide on GSH in the serum and tissues of mice (n = 4)
图 3 泡桐花多糖对小鼠血清和组织器官MDA的影响（n = 4）
Figure 3. Effect of Paulownia fortune flower polysaccharide on MDA in the serum and tissues of mice (n = 4)
图 4 泡桐花多糖对小鼠血清和组织器官T-AOC水平的影响（n = 4）
Figure 4. Effect of Paulownia fortune flower polysaccharide on T-AOC in the serum and tissues of mice (n = 4)
表 1 泡桐花多糖体外抗氧化指标测定
Table 1. Determination of antioxidant indexs in vitro of Paulownia fortune flower polysaccharide
组别 浓度（mg/mL） 超氧阴离子自由基清除率（%） 羟基自由基清除率（%） 抗H2O2氧化溶血率（%） 低剂量 0.5 54.36 ± 0.57d 74.62 ± 1.46c 6.96 ± 0.29c 中剂量 1 70.74 ± 1.82c 87.33 ± 1.36b 12.704 ± 0.43b 高剂量 2 81.10 ± 2.20b 93.65 ± 2.09a 15.97 ± 1.04a Vc 1 91.18 ± 2.25a 77.56 ± 2.40c 12.38 ± 0.54b 
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表 2 泡桐花多糖对雌性小鼠体重的影响（n = 5）
Table 2. Effect of Paulownia fortune flower polysaccharide on the weights of female mice (n = 5)
组别 浓度（mg/mL） 小鼠体体重 （g） D0 D7 D14 D21 D28 空白组 − 20.10 ± 0.87 24.88 ± 0.96 26.66 ± 1.02 28.24 ± 1.64 29.94 ± 1.68 低剂量 5 19.40 ± 0.79 24.36 ± 1.80 25.86 ± 2.41 26.90 ± 1.77 28.42 ± 1.36 中剂量 10 20.14 ± 1.25 24.58 ± 1.67 28.16 ± 1.97 28.30 ± 1.66 28.96 ± 3.39 高剂量 20 19.98 ± 1.22 24.70 ± 1.07 27.24 ± 0.63 27.66 ± 1.16 29.24 ± 2.08 Vc 10 19.36 ± 1.04 24.42 ± 2.31 26.78 ± 2.74 27.50 ± 2.97 28.72 ± 3.04
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表 3 泡桐花多糖对雄性小鼠体重的影响（n = 5）
Table 3. Effect of Paulownia fortune flower polysaccharide on the weights of male mice (n = 5)
组别 浓度（mg/mL） 小鼠体体重（g） D0 D7 D14 D21 D28 空白组 − 20.42 ± 1.40 26.98 ± 2.74 32.10 ± 3.14 34.12 ± 3.72 35.70 ± 3.49 低剂量 5 21.28 ± 1.05 26.68 ± 0.90 32.34 ± 1.43 34.46 ± 1.82 36.34 ± 2.13 中剂量 10 21.62 ± 0.34 26.36 ± 2.38 28.98 ± 3.26 31.32 ± 2.89 32.58 ± 3.29 高剂量 20 21.04 ± 1.38 27.32 ± 1.01 31.52 ± 1.72 33.74 ± 2.27 35.50 ± 2.86 Vc 10 20.16 ± 0.89 27.04 ± 1.47 30.98 ± 2.25 32.96 ± 2.16 34.74 ± 2.28
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