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中国精品科技期刊2020
王俊韵,沈静雯,陆利霞,等. 食源性病原菌的富集与检测复合技术研究进展[J]. 食品工业科技,2021,42(11):348−355. doi: 10.13386/j.issn1002-0306.2020060210.
引用本文: 王俊韵,沈静雯,陆利霞,等. 食源性病原菌的富集与检测复合技术研究进展[J]. 食品工业科技,2021,42(11):348−355. doi: 10.13386/j.issn1002-0306.2020060210.
WANG Junyun, SHEN Jingwen, LU Lixia, et al. Research Progress of Compound Techniques for Enrichment and Detection of Foodborne Pathogens [J]. Science and Technology of Food Industry, 2021, 42(11): 348−355. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060210.
Citation: WANG Junyun, SHEN Jingwen, LU Lixia, et al. Research Progress of Compound Techniques for Enrichment and Detection of Foodborne Pathogens [J]. Science and Technology of Food Industry, 2021, 42(11): 348−355. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060210.

食源性病原菌的富集与检测复合技术研究进展

Research Progress of Compound Techniques for Enrichment and Detection of Foodborne Pathogens

  • 摘要: 食源性致病菌对食品安全及人体健康造成威胁。食品中极低浓度的病原菌需要通过前处理再结合有效的检测技术才可检出。现有快速检测方法受到增菌液成分、食品成分或菌体浓度低的影响,需要进行致病菌的分离富集后才能缩短检测时间、进行准确检测。本文分析了单一的致病菌富集法,包括裸磁珠及功能化磁珠的富集法。进而综述了富集与检测一体法,包括电泳技术、微流控技术等。对于致病菌的特异性富集,需要进一步提高吸附率及灵敏度。富集检测复合技术的检测灵敏度及检测限有待不断改进。

     

    Abstract: Foodborne pathogen poses a threat to food safety and human health.The low concentrations pathogens in food can only be detected by pretreatment combined with effective detection techniques.The rapid detection methods are affected by the composition of the enrichment solution, food components, or low cell concentration, so it is necessary to separate and enrich the pathogenic bacteria from the samples in order to shorten the detection time and carry out accurate detection.In this paper, enrichment methods for pathogenic bacteria were analyzed, including bare magnetic beads and functional magnetic beads. Moreover, the integrated methods of enrichment and detection were reviewed, including electrophoresis technology, microfluidic technology and so on.For the enrichment of specific pathogenic bacteria, it is necessary to further improve the adsorption rate and sensitivity.It is continuously improved in detection sensitivity and detection limit for the completed technology of enrichment and detection.

     

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