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中国精品科技期刊2020

L-茶氨酸改善睡眠作用研究进展

张颖 王鑫 刘雅娟 马姗婕 杨月欣

张颖,王鑫,刘雅娟,等. L-茶氨酸改善睡眠作用研究进展[J]. 食品工业科技,2021,42(16):361−366. doi:  10.13386/j.issn1002-0306.2020070289
引用本文: 张颖,王鑫,刘雅娟,等. L-茶氨酸改善睡眠作用研究进展[J]. 食品工业科技,2021,42(16):361−366. doi:  10.13386/j.issn1002-0306.2020070289
ZHANG Ying, WANG Xin, LIU Yajuan, et al. Research Progress on Improving Sleep Mechanism of L-theanine[J]. Science and Technology of Food Industry, 2021, 42(16): 361−366. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020070289
Citation: ZHANG Ying, WANG Xin, LIU Yajuan, et al. Research Progress on Improving Sleep Mechanism of L-theanine [J]. Science and Technology of Food Industry, 2021, 42(16): 361−366. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020070289

L-茶氨酸改善睡眠作用研究进展

doi: 10.13386/j.issn1002-0306.2020070289
详细信息
    作者简介:

    张颖(1995−),女,硕士研究生,研究方向:食品营养与健康,E-mail:zying18@mails.jlu.edu.cn

    通讯作者:

    马姗婕(1987−),女,硕士,工程师,研究方向:功能食品评估评价,E-mail:mashanjie@hotmail.com

    杨月欣(1955−),女,博士,教授,研究方向:食品营养与健康,E-mail:yxyang@cnsoc.org

  • 中图分类号: TS201.4

Research Progress on Improving Sleep Mechanism of L-theanine

  • 摘要: L-茶氨酸(L-Theanine)是茶叶中特有的一种非蛋白氨基酸,具有保护神经、降血压、抗氧化、增强机体免疫、改善认知等多种生理活性作用。因其具有镇静安神的特性,L-茶氨酸在改善睡眠方面的研究逐渐增多。本文就L-茶氨酸的代谢过程及其改善睡眠的作用机制的相关研究进行综述,旨在为L-茶氨酸在改善睡眠方面的进一步研究和L-茶氨酸的进一步开发利用提供参考。
  • 图  1  L-茶氨酸、L-谷氨酸和L-谷氨酰胺的化学结构式

    Figure  1.  Chemical structural formulas of L-theanine, L-glutamic acid and L-glutamine

    图  2  L-茶氨酸影响谷氨酸的可能机制

    Figure  2.  Possible mechanism of L-theanine affecting glutamic acid

    注:①L-茶氨酸(L-THE)竞争性抑制Gln与ASCT2受体结合;②L-茶氨酸(L-THE)竞争性抑制Glu与谷氨酸受体(NMDA、AMPA、Ka)结合 。

    表  1  L-茶氨酸的改善睡眠作用研究

    Table  1.   Summary of studies on sleep improvement effect of L-theanine

    受试对象年龄研究设计干预剂量结论参考
    93例诊断为ADHD
    的儿童
    8~12岁随机,双盲,安慰剂对照的临床试验400 mg/d睡眠效率显著提高,夜间活动减少。Lyon等[25]
    46例诊断为GAD
    的患者
    18~75岁随机,双盲,安慰剂对照(8周)450 mg/dGAD患者自我评价的睡眠满意度显著高于安慰剂组,且部分GAD患者的失眠症状得到了改善。Sarris等[26]
    20例诊断为MDD
    的患者
    20~67岁开放标签研究(8周)250 mg/dPSQI相比基线水平显著降低。Hidese等[27]
    17例诊断为
    精神分裂的患者
    28~53岁开放标签研究(8周)250 mg/d主观睡眠质量(根据PSQI)有所改善。Ota等[28]
    68例诊断为失眠症
    的老年患者
    62~81岁随机,安慰剂对照的临床试验1800 mg/dPSQI各得分显著下降,患者入睡时间明显缩短,睡眠持续时间明显延长,睡眠效率提高,觉醒后日间功能改善的人数明显增加。陈玲夫[29]
    22例健康男性27.5±0.9岁随机,双盲,安慰剂对照,交叉试验200 mg/d睡眠比例显著增加,WASO显著降低。Ozeki等[30]
    20例绝经后妇女57.3±3.9岁随机,双盲,安慰剂对照,交叉试验200 mg/d显着增加睡眠前半部分的PNA并降低睡眠后半部分的SNA。Ozeki等[31]
    30例健康个体48.3±11.9岁随机,双盲,安慰剂对照,交叉试验(四周)200 mg/d睡眠潜伏期,睡眠障碍和睡眠药物使用的PSQI量表得分显著降低。Hidese等[33]
    8只成年SD雄性大鼠/根据分组腹腔注射生理盐水、咖啡因或茶氨酸,植入电极监测睡眠状况22.5、37.5、75或
    150 mg/kg
    L-茶氨酸可以减轻咖啡因诱导的大鼠SWS的降低,且减轻作用不随剂量增加而增强。Jang等[11]
    8只雄性ICR小鼠4周龄腹腔注射戊巴比妥钠建立睡眠模型,观测睡眠潜伏期和总睡眠时间20、30、40 mg/kg20 mg/kg的L-茶氨酸组睡眠潜伏期减少和总睡眠时间增加,30 mg/kg组的睡眠潜伏期减少。Kim等[34]
    8只SD大鼠8周龄腹腔注射戊巴比妥钠建立睡眠模型,脑内植入电极监测睡眠状况20 mg/kgREM时间和θ波显著增加。Kim等[34]
    8只SD大鼠8周龄口服咖啡因建立觉醒模型20 mg/kgL-茶氨酸可将NREM睡眠时间恢复至正常水平。Kim等[34]
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  • [1] Xie Z, Chen F, Li W A, et al. A review of sleep disorders and melatonin[J]. Neurol Res,2017,39(6):559−565. doi:  10.1080/01616412.2017.1315864
    [2] 裴清华, 李侠, 许静, 等. 308例原发性失眠患者的中医体质分布与睡眠质量的相关性研究[J]. 北京中医药大学学报,2018,41(10):859−862. doi:  10.3969/j.issn.1006-2157.2018.10.012
    [3] Yuen K M, Pelayo R. Socioeconomic impact of pediatric sleep disorders[J]. Sleep Med Clin,2017,12(1):23−30. doi:  10.1016/j.jsmc.2016.10.005
    [4] Wickwire E M, Shaya F T, Scharf S M. Health economics of insomnia treatments: The return on investment for a good night's sleep[J]. Sleep Med Rev,2016,30:72−82. doi:  10.1016/j.smrv.2015.11.004
    [5] Doghramji K, Jangro W C. Adverse effects of psychotropic medications on sleep[J]. Sleep Med Clin,2016,11(4):503−514. doi:  10.1016/j.jsmc.2016.08.001
    [6] Graham H N. Green tea composition, consumption, and polyphenol chemistry[J]. Prev Med,1992,21(3):334−350. doi:  10.1016/0091-7435(92)90041-F
    [7] Cicero A F G, Fogacci F, Banach M. Botanicals and phytochemicals active on cognitive decline: The clinical evidence[J]. Pharmacol Res,2018,130:204−212. doi:  10.1016/j.phrs.2017.12.029
    [8] Li C, Tong H, Yan Q, et al. L-theanine improves immunity by altering TH2/TH1 cytokine balance, brain neurotransmitters, and expression of phospholipase C in rat hearts[J]. Med Sci Monit,2016,22:662−669. doi:  10.12659/MSM.897077
    [9] Lopes Sakamoto F, Metzker Pereira Ribeiro R, Amador Bueno A, et al. Psychotropic effects of L-theanine and its clinical properties: From the management of anxiety and stress to a potential use in schizophrenia[J]. Pharmacol Res,2019,147:104395. doi:  10.1016/j.phrs.2019.104395
    [10] Lin L, Zeng L, Liu A, et al. L-theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways[J]. Food Funct,2020,11(2):1798−1809. doi:  10.1039/C9FO02451D
    [11] Jang H S, Jung J Y, Jang I S, et al. L-theanine partially counteracts caffeine-induced sleep disturbances in rats[J]. Pharmacol Biochem Behav,2012,101(2):217−221. doi:  10.1016/j.pbb.2012.01.011
    [12] 李靓, 林智, 郭丽, 等. 茶氨酸改善小鼠睡眠状况的实验研究[J]. 食品科学,2009,30(15):214−216. doi:  10.3321/j.issn:1002-6630.2009.15.050
    [13] 陈子彤, 陈希民, 陈月晓, 等. 酸枣仁、茶氨酸、γ-氨基丁酸联合使用改善睡眠作用研究[J]. 食品科技,2020,45(6):81−85.
    [14] 李成舰, 罗乐, 黄春花. L-茶氨酸抗氧化作用的研究进展[J]. 食品科学技术学报,2018,36(4):69−75. doi:  10.3969/j.issn.2095-6002.2018.04.010
    [15] Deb S, Dutta A, Phukan B C, et al. Neuroprotective attributes of L-theanine, a bioactive amino acid of tea, and its potential role in Parkinson ’s disease therapeutics[J]. Neurochem Int,2019,129:104478. doi:  10.1016/j.neuint.2019.104478
    [16] Yamamoto S, Kimura T, Tachiki T, et al. The involvement of L-type amino acid transporters in theanine transport[J]. Biosci Biotechnol Biochem,2012,76(12):2230−2235. doi:  10.1271/bbb.120519
    [17] Yokogoshi H, Kobayashi M, Mochizuki M, et al. Effect of theanine, r-glutamylethylamide, on brain monoamines and striatal dopamine release in conscious rats[J]. Neurochem Res,1998,23(5):667−673. doi:  10.1023/A:1022490806093
    [18] Unno T, Suzuki Y, Kakuda T, et al. Metabolism of theanine, gamma-glutamylethylamide, in rats[J]. J Agric Food Chem,1999,47(4):1593−1596. doi:  10.1021/jf981113t
    [19] Vuong Q V, Bowyer M C, Roach P D. L-theanine: properties, synthesis and isolation from tea[J]. J Sci Food Agric,2011,91(11):1931−1939. doi:  10.1002/jsfa.4373
    [20] Scheid L, Ellinger S, Alteheld B, et al. Kinetics of L-theanine uptake and metabolism in healthy participants are comparable after ingestion of L-theanine via capsules and green tea[J]. J Nutr,2012,142(12):2091−2096. doi:  10.3945/jn.112.166371
    [21] Pijl P, Chen L, Mulder T. Human disposition of L-theanine in tea or aqueous solution[J]. Journal of Functional Foods,2010,2(4):239−244. doi:  10.1016/j.jff.2010.08.001
    [22] Amihaesei I C, Mungiu O C. Main neuroendocrine features and therapy in primary sleep troubles[J]. Rev Med Chir Soc Med Nat Iasi,2012,116(3):862−866.
    [23] Dopheide J A. Insomnia overview: Epidemiology, pathophysiology, diagnosis and monitoring, and nonpharmacologic therapy[J]. Am J Manag Care,2020,26(4 Suppl):S76−S84.
    [24] Mason B L, Davidov A, Minhajuddin A, et al. Focusing on insomnia symptoms to better understand depression: A STAR*D report[J]. J Affect Disord,2020,260:183−186. doi:  10.1016/j.jad.2019.08.094
    [25] Lyon M R, Kapoor M P, Juneja L R. The effects of L-theanine (suntheanine(R)) on objective sleep quality in boys with attention deficit hyperactivity disorder (ADHD): A randomized, double-blind, placebo-controlled clinical trial[J]. Altern Med Rev,2011,16(4):348−354.
    [26] Sarris J, Byrne G J, Cribb L, et al. L-theanine in the adjunctive treatment of generalized anxiety disorder: A double-blind, randomised, placebo-controlled trial[J]. J Psychiatr Res,2019,110:31−37. doi:  10.1016/j.jpsychires.2018.12.014
    [27] Hidese S, Ota M, Wakabayashi C, et al. Effects of chronic L-theanine administration in patients with major depressive disorder: An open-label study[J]. Acta Neuropsychiatr,2017,29(2):72−79. doi:  10.1017/neu.2016.33
    [28] Ota M, Wakabayashi C, Sato N, et al. Effect of L-theanine on glutamatergic function in patients with schizophrenia[J]. Acta Neuropsychiatr,2015,27(5):291−296. doi:  10.1017/neu.2015.22
    [29] 陈玲夫. 茶氨酸胶囊干预老年失眠症的临床疗效观察[J]. 海峡药学,2011,23(6):117−119. doi:  10.3969/j.issn.1006-3765.2011.06.055
    [30] Ozeki M, Juneja L R, Shirakawa S. The effects of L-theanine on sleep using the actigaph[J]. Japanese Journal of Physiological Anthropology,2004,9(4):143−150.
    [31] Ozeki M, Juneja L R, Shirakawa S. The effects of L-theanine on automatic nervous system during sleep in postmenopausal women[J]. Japanese Journal of Physiological Anthropology,2008,13(3):147−154.
    [32] Rao T P, Ozeki M, Juneja L R. In search of a safe natural sleep aid[J]. J Am Coll Nutr,2015,34(5):436−447. doi:  10.1080/07315724.2014.926153
    [33] Hidese S, Ogawa S, Ota M, et al. Effects of L-theanine administration on stress-related symptoms and cognitive functions in healthy adults: A randomized controlled trial[J]. Nutrients,2019,11(10):2362. doi:  10.3390/nu11102362
    [34] Kim S, Jo K, Hong K B, et al. GABA and L-theanine mixture decreases sleep latency and improves NREM sleep[J]. Pharm Biol,2019,57(1):65−73.
    [35] Gottesmann C. GABA mechanisms and sleep[J]. Neuroscience,2002,111(2):231−239. doi:  10.1016/S0306-4522(02)00034-9
    [36] Sharma E, Joshi R, Gulati A. L-theanine: An astounding sui generis integrant in tea[J]. Food Chem,2018,242:601−610. doi:  10.1016/j.foodchem.2017.09.046
    [37] Kimura R, Murata T. Influence of alkylamides of glutamic acid and related compounds on the central nervous system. I. Central depressant effect of theanine[J]. Chem Pharm Bull (Tokyo),1971,19(6):1257−1261. doi:  10.1248/cpb.19.1257
    [38] Hageman I, Andersen H S, Jørgensen M B. Post-traumatic stress disorder: A review of psychobiology and pharmacotherapy[J]. Acta Psychiatr Scand,2001,104(6):411−422. doi:  10.1034/j.1600-0447.2001.00237.x
    [39] 辛静. 刺人参改善睡眠作用的有效部位的筛选及其作用机制研究[D]. 哈尔滨: 黑龙江中医药大学, 2012.
    [40] Yamada T, Terashima T, Okubo T, et al. Effects of theanine, r-glutamylethylamide, on neurotransmitter release and its relationship with glutamic acid neurotransmission[J]. Nutritional Neuroscience,2005,8(4):219−226. doi:  10.1080/10284150500170799
    [41] Kakuda T, Hinoi E, Abe A, et al. Theanine, an ingredient of green tea, inhibits [3H]glutamine transport in neurons and astroglia in rat brain[J]. J Neurosci Res,2008,86(8):1846−1856. doi:  10.1002/jnr.21637
    [42] Kakuda T. Neuroprotective effects of theanine and its preventive effects on cognitive dysfunction[J]. Pharmacol Res,2011,64(2):162−168. doi:  10.1016/j.phrs.2011.03.010
    [43] Liu W L, Wu B F, Shang J H, et al. Moringa oleifera lam seed oil augments pentobarbital-induced sleeping behaviors in mice via GABAergic systems[J]. J Agric Food Chem,2020,68(10):3149−3162. doi:  10.1021/acs.jafc.0c00037
    [44] Li N, Liu J, Wang M, et al. Sedative and hypnotic effects of schisandrin B through increasing GABA/Glu ratio and upregulating the expression of GABAA in mice and rats[J]. Biomed Pharmacother,2018,103:509−516. doi:  10.1016/j.biopha.2018.04.017
    [45] Inoue K, Miyazaki Y, Unno K, et al. Stable isotope dilution HILIC-MS/MS method for accurate quantification of glutamic acid, glutamine, pyroglutamic acid, GABA and theanine in mouse brain tissues[J]. Biomed Chromatogr,2016,30(1):55−61. doi:  10.1002/bmc.3502
    [46] Juneja L R, Chu D C, Okubo T, et al. L-theanine-a unique amino acid of green tea and its relaxation effect in humans[J]. Trends Food Sci Tech,1999,10(6-7):199−204. doi:  10.1016/S0924-2244(99)00044-8
    [47] Turkozu D, Sanlier N. L-theanine, unique amino acid of tea, and its metabolism, health effects, and safety[J]. Crit Rev Food Sci Nutr,2017,57(8):1681−1687. doi:  10.1080/10408398.2015.1016141
    [48] Kobayashi K, Nagato Y, Aoi N, et al. Effects of L-theanine on the release of alpha-brain waves in human volunteers[J]. Nippon Nogeik Kaishi,1998,72(2):153−157. doi:  10.1271/nogeikagaku1924.72.153
    [49] Kim K S, Song C H, Oh H J. Effects of theanine on the release of brain alpha wave in adult males[J]. Faseb Journal,2004,18(4):A541−A542.
    [50] Micallef J, Soubrouillard C, Guet F, et al. A double blind parallel group placebo controlled comparison of sedative and mnesic effects of etifoxine and lorazepam in healthy subjects [corrected][J]. Fundam Clin Pharmacol,2001,15(3):209−216. doi:  10.1046/j.1472-8206.2001.00025.x
    [51] M Ishidate, T Sofuni, K Yoshikawa, et al. Primary mutagenicity screening of food additives currently used in Japan[J]. Food and Chemical Toxicology,1984,22(8):623−636. doi:  10.1016/0278-6915(84)90271-0
    [52] S Fujii, K Inai. Tumorigenicity study of L-theanine administrated orally to mice[J]. Food Chemistry,2008,110(3):643−646. doi:  10.1016/j.foodchem.2008.02.052
    [53] 刘洋, 李颂, 王春玲. 茶氨酸健康功效研究进展[J]. 食品研究与开发,2016,37(17):211−214. doi:  10.3969/j.issn.1005-6521.2016.17.052
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  • 收稿日期:  2020-07-24
  • 网络出版日期:  2021-07-05
  • 刊出日期:  2021-08-15

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