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中国精品科技期刊2020

阿拉伯半乳聚糖对2型糖尿病大鼠肠道碱性磷酸酶、细菌内毒素和肠道中细胞因子的影响

王语聪 谢智鑫 张学艳 赵太云 VladimirOstronkov LokhovDmitrii 韩建春

王语聪,谢智鑫,张学艳,等. 阿拉伯半乳聚糖对2型糖尿病大鼠肠道碱性磷酸酶、细菌内毒素和肠道中细胞因子的影响[J]. 食品工业科技,2021,42(14):334−340. doi:  10.13386/j.issn1002-0306.2020090167
引用本文: 王语聪,谢智鑫,张学艳,等. 阿拉伯半乳聚糖对2型糖尿病大鼠肠道碱性磷酸酶、细菌内毒素和肠道中细胞因子的影响[J]. 食品工业科技,2021,42(14):334−340. doi:  10.13386/j.issn1002-0306.2020090167
WANG Yucong, XIE Zhixin, ZHANG Xueyan, et al. Effect of Arabinogalactan on Intestinal Alkaline Phosphatase, Bacterial Endotoxin and Serum Cytokines in Type 2 Diabetic Rats[J]. Science and Technology of Food Industry, 2021, 42(14): 334−340. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020090167
Citation: WANG Yucong, XIE Zhixin, ZHANG Xueyan, et al. Effect of Arabinogalactan on Intestinal Alkaline Phosphatase, Bacterial Endotoxin and Serum Cytokines in Type 2 Diabetic Rats [J]. Science and Technology of Food Industry, 2021, 42(14): 334−340. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020090167

阿拉伯半乳聚糖对2型糖尿病大鼠肠道碱性磷酸酶、细菌内毒素和肠道中细胞因子的影响

doi: 10.13386/j.issn1002-0306.2020090167
基金项目: 黑龙江绿色食品科学研究院(2020230001000591)
详细信息
    作者简介:

    王语聪(1994−),女,硕士研究生,研究方向:农产品加工及贮藏,E-mail:wangyucong1115@163.com

    通讯作者:

    韩建春(1973−),男,博士,教授,研究方向:农产品加工及贮藏,E-mail:hanjianchun@hotmail.com

  • 中图分类号: TS201.4

Effect of Arabinogalactan on Intestinal Alkaline Phosphatase, Bacterial Endotoxin and Serum Cytokines in Type 2 Diabetic Rats

  • 摘要: 本实验以低剂量腹腔注射链脲佐菌素(30 mg/kg)建立糖尿病大鼠模型,建模成功后,连续给予大鼠灌胃阿拉伯半乳聚糖(Arabinogalactan, AG)水溶液(100 mg/kg、500 mg/kg),测定大鼠体质量及血糖变化、粪便中肠道碱性磷酸酶(IAP)的活力及细菌内毒素中脂多糖(LPS)的浓度变化,并通过MSD多因子方法检测肠道中细胞因子浓度的变化。结果表明,与模型组大鼠相比,AG剂量组空腹血糖浓度显著降低(P<0.05),IAP活力显著增加(P<0.05),LPS浓度显著降低(P<0.05),且肠道中促炎因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素-1(IL-1β)、生长调节致癌基因α(KC/GRO)的浓度显著减少(P<0.05),而抗炎因子白介素-4(IL-4)、白介素-5(IL-5)、白介素-10(IL-10)、白介素-13(IL-13)的浓度显著增加(P<0.05)。因此,可以证明AG能够提高IAP活力,降低LPS浓度,调控大鼠细胞因子,进而对2型糖尿病大鼠模型在免疫方面具有一定改善作用。
  • 图  1  各组大鼠体质量随干预时间变化($\bar {\rm{x}} \pm {\rm{s}} $, n=10)

    Figure  1.  Changes in body weight of rats in each group with the time of intervention ($\bar {\rm{x}} \pm {\rm{s}} $, n=10)

    注:*表示与正常对照组相比,P<0.05;#表示与模型组相比,P<0.05。

    图  2  AG对大鼠肠液IL-6、IL-1β、TNF-α和IFN-γ浓度的影响($\bar {\rm{x}} \pm {\rm{s}}$,n=10)

    Figure  2.  Arabinogalactan on the concentration of IL-6, IL-1β, TNF-α and IFN-γ in mouse serum ($\bar {\rm{x}} \pm {\rm{s}}$, n=10)

    注:*:与正常对照组相比,P<0.05;**:与正常对照组相比,P<0.01;#:与模型组相比,P<0.05;##:与模型组相比,P<0.01;图3~图4同。

    图  3  AG对大鼠肠液IL-4、IL-5、IL-10和IL-13浓度的影响($\bar {\rm{x}} \pm {\rm{s}}$, n=10)

    Figure  3.  Arabinogalactan on the concentration of IL-4, IL-5, IL-10 and IL-13 in mouse serum ($\bar {\rm{x}} \pm {\rm{s}}$, n=10)

    图  4  AG对大鼠肠液KC/GRO浓度的影响($\bar {\rm{x}} \pm {\rm{s}}$, n=10)

    Figure  4.  Arabinogalactan affects the serum KC/GRO concentration of mice ($\bar {\rm{x}} \pm {\rm{s}}$, n=10)

    表  1  AG对2型糖尿病大鼠空腹血糖的影响($\bar {\rm{x}} \pm {\rm{s}} $, n=10)

    Table  1.   Effect of arabinogalactan on fasting blood glucose in type 2 diabetic rats ($\bar {\rm{x}} \pm {\rm{s}} $, n=10)

    组别血糖含量(mmol/L)
    0 d7 d14 d21 d28 d
    正常对照组5.6800±0.13025.8000±0.14415.3000±0.82806.2000±0.7010c5.6000±0.5940
    模型组5.9000±0.310616.2000±0.7219**15.0000±2.5700**15.6000±1.7380**16.1000±1.7040**
    AG低剂量组5.6733±0.151216.9000±0.796715.7000±1.522014.3000±2.520012.5000±1.5540#
    AG高剂量组5.7500±0.255216.5000±3.124014.5000±2.775013.4000±2.5290#11.2000±1.7100#
    注:*表示与正常对照组相比,P<0.05;**表示与正常对照组相比,P<0.01;#表示与模型组相比,P<0.05;##表示与模型组相比,P<0.01;表2同。
    下载: 导出CSV

    表  2  AG对2型糖尿病大鼠粪便中IAP和LPS影响($\bar {\rm{x}} \pm {\rm{s}} $, n=10)

    Table  2.   Effect of arabinogalactan powder on IAP and IAP in fecal of type 2 diabetic rats ($\bar {\rm{x}} \pm {\rm{s}} $, n=10)

    组别LPS(EU/mL)IAP活性(U)
    正常对照组3.664±0.20187.550±0.387
    模型组7.813±0.4364*31.367±0.469**
    AG低剂量组2.270±0.081#94.668±1.807##
    AG高剂量组2.321±0.0329#95.707±0.388##
    下载: 导出CSV
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  • 收稿日期:  2020-09-16
  • 网络出版日期:  2021-06-08
  • 刊出日期:  2021-07-07

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