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中国精品科技期刊2020

贵定云雾茶本地种和引进种次生代谢产物差异分析

王春波 吕辉 韦玲冬 郭治友

王春波,吕辉,韦玲冬,等. 贵定云雾茶本地种和引进种次生代谢产物差异分析[J]. 食品工业科技,2021,42(14):1−7. doi:  10.13386/j.issn1002-0306.2020110282a
引用本文: 王春波,吕辉,韦玲冬,等. 贵定云雾茶本地种和引进种次生代谢产物差异分析[J]. 食品工业科技,2021,42(14):1−7. doi:  10.13386/j.issn1002-0306.2020110282a
WANG Chunbo, LYU Hui, WEI Lingdong, et al. Analysis on Secondary Metabolites Difference of Guiding Yunwu Tea between Native and Introduced Varieties[J]. Science and Technology of Food Industry, 2021, 42(14): 1−7. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020110282a
Citation: WANG Chunbo, LYU Hui, WEI Lingdong, et al. Analysis on Secondary Metabolites Difference of Guiding Yunwu Tea between Native and Introduced Varieties[J]. Science and Technology of Food Industry, 2021, 42(14): 1−7. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020110282a

贵定云雾茶本地种和引进种次生代谢产物差异分析

doi: 10.13386/j.issn1002-0306.2020110282a
基金项目: 国家自然科学基金项目(31660056);贵州省教育厅青年科技人才成长项目(黔教合KY字[2020]211);贵州省科技计划项目(黔科合平台人[2020]QNSYXM02)
详细信息
    作者简介:

    王春波(1987−),男,博士,副教授,研究方向:植物代谢组学,Email:wchunb@mail2.sysu.edu.cn

  • 中图分类号: Q941.2

Analysis on Secondary Metabolites Difference of Guiding Yunwu Tea between Native and Introduced Varieties

  • 摘要: 为了研究贵定云雾茶本地种和引进种次生代谢产物差异,基于超高效液相色谱-四级杆串联飞行时间质谱(ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry, UPLC-QTOF-MS)对贵定云雾茶本地种和引进种的次生代谢产物进行了定性、定量分析。结果鉴定出361种代谢物。采用主成分分析(principal component analysis, PCA)和正交偏最小二乘判别分析(orthogonal least squares discriminant analysis, OPLS-DA)筛选出14种显著差异代谢物,包括4种黄酮醇类物质、4种酚酸类物质、3种黄酮糖苷类物质、2种儿茶素和1种原花青素B1。通路富集分析显示这些差异代谢物主要分布于苯丙氨酸、酪氨酸和色氨酸代谢途径(phenylalanine, tyrosine and tryptophan biosynthesis)、黄酮和黄酮醇代谢途径(flavone and flavonol biosynthesis)以及类黄酮代谢途径中(flavonoid biosynthesis)。此外,儿茶素和原花青素B1在云雾茶本地种中含量较高,而黄酮醇和黄酮糖苷类物质在引进种中含量较高,这表明云雾茶本地种更适合绿茶的加工,而引进种更适合白茶的加工。
  • 图  1  样品负离子模式下基峰图

    Figure  1.  Base peak map of samples in negative mode

    注:A:本地种;B:引进种。

    图  2  主成分分析和正交偏最小二乘判别分析

    Figure  2.  Principal component analysis and orthogonal least squares discriminant analysis

    图  3  正交偏最小二乘判别分析载荷图

    Figure  3.  Loading plot of orthogonal least squares discriminant analysis

    注:代谢物编号对应表1

    图  4  差异代谢物热图

    Figure  4.  Heat map of differential metabolites between two varieties

    图  5  苯丙氨酸、酪氨酸和色氨酸代谢途径

    Figure  5.  Phenylalanine, tyrosine and tryptophan biosynthesis

    注:加粗的为差异代谢物,柱状图代表物质相对丰度;图6同。

    图  6  黄酮和黄酮醇代谢途径以及类黄酮代谢途径

    Figure  6.  Flavone and flavonol biosynthesis, and flavnoid biosynthesis

    表  1  不同云雾茶树品种的差异代谢物

    Table  1.   Differencial metabolites between two varieties

    编号代谢物名称分子式保留时间(min)质荷比(m/z)VIP值倍数值log2(FC)
    1Myriletin杨梅素C15H10O83.47319.044396.253.61
    2Dihydroquercetin二氢槲皮素C15H12O73.59303.050661.851.29
    3Quercetin槲皮素C15H10O74.15301.049539.474.27
    4Kaempferol山奈酚C15H10O64.27287.054673.818.36
    5Quinic acid奎尼酸C7H12O60.46191.0557821.92−1.91
    6Phenylpyruvic acid苯丙酮酸C9H8O30.95163.039952.26−4.24
    7Caffeoyl quinic acid咖啡酰奎宁酸C16H18O92.67353.087472.012.07
    8Hydroxybenzoic acid羟基苯甲酸C7H6O33.33137.023801.361.98
    9Quercetin-3-O-hexosyl(1-2)
    deoxyhexosyl-7-O-hexoside
    槲皮素-己糖苷C41H70O133.41771.1980812.445.63
    10Kaempferol-3-Rhamnoside-7-Rhamnoside山奈酚-鼠李糖苷C27H30O143.50755.2030710.392.06
    11Kaempferol-3-Galactoside-6-
    Rhamnoside-3-Rhamnoside
    山奈酚-半乳糖苷-鼠李糖苷C33H40O193.61739.208365.53−8.03
    12Epigallocatechin表没食子儿茶素C15H14O710.72305.066391.871.91
    13Epicatechin表儿茶素C15H14O63.32289.0714425.452.41
    14Procyanidin B1原花青素B1C30H26O123.18577.134553.14−1.68
    注:表中log2(FC)表示对代谢物在本地种和引进种中含量的倍数值求log2的对数。
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-11-30
  • 网络出版日期:  2021-05-27
  • 刊出日期:  2021-07-07

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