Abstract: Based on the metabolomic technique of GC-MS, the metabolites and metabolic differential mechanism of rice from Ning'an and Wuchang city were studied. SIMCA and R software were used for multivariate statistical analysis of the data. The results showed that the place of production had an influence on the quantity, content, type and proportion of the metabolites of Daohuaxiang rice. A total of 127 metabolites were identified in rice samples from the two regions. A total of 22 different metabolites were screened out through the analysis of the differences between the samples from the two regions. And it was found that the origin had the most significant influence on the contents of fatty acids and their derivatives. The results of metabolic pathway analysis showed that the main mechanism of rice metabolism difference in different producing areas was the metabolism of fatty acids. Linoleic acid, palmitic acid and fumaric acid were the three key metabolites. And the difference of metabolites type and content was related to rice quality. The research analyzes the different mechanisms of rice metabolism in different producing areas from the aspect of metabolite composition, which can provide theoretical support for the regional differentiation of rice production and the traceability of agricultural products.